| Integration of a two-phase partition method into proteomics research on rat liver plasma membrane proteins. | |
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MedLine Citation:
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PMID: 16512679 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To comprehensively identify proteins of the rat liver plasma membrane (PM), we have adopted a proteomics strategy that utilizes sucrose density centrifugation in conjunction with aqueous two-phase partition for plasma membrane isolation, followed by SDS-PAGE, mass spectrometry and bioinformatics. Western blot analysis showed that this method results in highly purified plasma membrane fractions, which is a key to successful plasma membrane proteomics. The PM proteins were separated by SDS-PAGE and digested with trypsin. Through nano-ESI-LC MS/MS analysis we identified 428 rat liver membrane proteins, of which 304 had a gene ontology (GO) annotation indicating a cellular component, and 204 (67%) of the latter were known integral membrane proteins or membrane-associated proteins. In addition to proteins known to be associated with the plasma membrane, several hypothetical proteins have also been identified. This study not only provides a tool to study plasma membrane proteins with low levels of contamination, but also provides a data set for proteins of high to moderate abundance in rat liver plasma membranes, thus allowing for more comprehensive characterization of membrane proteins and a better understanding of membrane dynamics. |
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Authors:
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Rui Cao; Xuanwen Li; Zhen Liu; Xia Peng; Weijun Hu; Xianchun Wang; Ping Chen; Jingyun Xie; Songping Liang |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of proteome research Volume: 5 ISSN: 1535-3893 ISO Abbreviation: J. Proteome Res. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-03-03 Completed Date: 2007-03-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101128775 Medline TA: J Proteome Res Country: United States |
Other Details:
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Languages: eng Pagination: 634-42 Citation Subset: IM |
Affiliation:
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Key Laboratory of Protein Chemistry and Developmental Biology of Education Committee, College of Life Sciences, Hunan Normal University, Changsha, People's Republic of China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Membrane / chemistry, enzymology, metabolism* Gene Expression Regulation Liver / enzymology, metabolism* Membrane Proteins / chemistry, isolation & purification, metabolism* Proteomics / methods* Rats Signal Transduction / physiology |
| Chemical | |
Reg. No./Substance:
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0/Membrane Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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