Document Detail


Integration and excision of pMC7105 in Pseudomonas syringae pv. phaseolicola: involvement of repetitive sequences.
MedLine Citation:
PMID:  6321436     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The site for integration of pMC7105 into the chromosome of Pseudomonas syringae pv. phaseolicola has been mapped to a 2.6-kilobase-pair (kb) Bg/II-EcoRI fragment on this 150-kb indigenous plasmid. Selected excision plasmids resulting from imprecise excision of pMC7105 were used to identify one of the plasmid-chromosome juncture fragments and to characterize the mechanism of recombination from the chromosome. A 14.2-kb BamHI plasmid-chromosome juncture fragment has been identified in pEX8060 (234 kb), an excision plasmid which carries approximately 90 kb of chromosomal sequences to the left of the site of integration. This fragment contains a portion of the 2.6-kb Bg/II-EcoRI fragment as well as chromosomal sequences. Blot hybridization with a probe made from selected fragments of pMC7105 revealed three distinct repetitive sequences, RS-I, RS-II, and RS-III, on this plasmid. The 2.6-kb fragment, to which the site of integration maps, also contains RS-II. Five copies of RS-II are present in pMC7105, and more than 20 copies are present in the chromosome. Eight small excision plasmids were shown to result from recombination among fragments of pMC7105 that contain common repetitive sequences. The results indicate that integration and excision of pMC7105 occur through general recombination at homologous repetitive sequences.
Authors:
L J Szabo; D Mills
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  157     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1984 Mar 
Date Detail:
Created Date:  1984-04-11     Completed Date:  1984-04-11     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  821-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Chromosomes, Bacterial*
DNA Restriction Enzymes
DNA, Bacterial
Deoxyribonuclease BamHI
Nucleic Acid Hybridization
Plasmids*
Pseudomonas / genetics*,  ultrastructure
Recombination, Genetic*
Repetitive Sequences, Nucleic Acid*
Chemical
Reg. No./Substance:
0/DNA, Bacterial; EC 3.1.21.-/DNA Restriction Enzymes; EC 3.1.21.-/Deoxyribonuclease BamHI
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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