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Integration of Metabolomics and Subcellular Organelle Expression Microarray Increases Understanding of the Organic Acid Changes in Post-harvest Citrus Fruit.
MedLine Citation:
PMID:  23758915     Owner:  NLM     Status:  Publisher    
Citric acid plays an important role in fresh fruit flavor and its adaptability to post-harvest storage conditions. In order to explore organic acid regulatory mechanisms in post-harvest citrus fruit, systematic biological analyses were conducted on stored Hirado Buntan Pummelo (HBP) (Citrus grandis) fruits. High-performance capillary electrophoresis (HPCE), subcellular organelle expression microarray, real-time quantitative reverse-transcription PCR (qRT-PCR), gas chromatography-mass spectrometry (GC-MS) and conventional physiological and biochemical analyses were undertaken. The results showed that the concentration of organic acids in HBP underwent a regular fluctuation. GC-MS based metabolic profiling indicated that succinic acid, γ-aminobutyric acid (GABA) and glutamine contents increased, but 2-oxoglutaric acid content declined, which further confirmed that the GABA shunt may have some regulatory roles in organic acid catabolism processes. In addition, the concentration of organic acids was significantly correlated with senescence-related physiological processes, such as hydrogen peroxide (H2 O2 ) content as well as superoxide dismutase (SOD) and perioxidase (POD) activities, which showed that organic acids could be regarded as important parameters for measuring citrus fruit post-harvest senescence processes.
Xiaohua Sun; Andan Zhu; Shuzhen Liu; Ling Sheng; Qiaoli Ma; Li Zhang; Elsayed Mohamed Elsayed Nishawy; Yunliu Zeng; Juan Xu; Zhaocheng Ma; Yunjiang Cheng; Xiuxin Deng
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-6-11
Journal Detail:
Title:  Journal of integrative plant biology     Volume:  -     ISSN:  1744-7909     ISO Abbreviation:  J Integr Plant Biol     Publication Date:  2013 Jun 
Date Detail:
Created Date:  2013-6-13     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101250502     Medline TA:  J Integr Plant Biol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
This article is protected by copyright. All rights reserved.
Key Laboratory of Horticultural Plant Biology, Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
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