Document Detail

Integrating qPLM and biomechanical test data with an anisotropic fiber distribution model and predictions of TGF-β1 and IGF-1 regulation of articular cartilage fiber modulus.
MedLine Citation:
PMID:  23266906     Owner:  NLM     Status:  MEDLINE    
A continuum mixture model with distinct collagen (COL) and glycosaminoglycan elastic constituents was developed for the solid matrix of immature bovine articular cartilage. A continuous COL fiber volume fraction distribution function and a true COL fiber elastic modulus ([Formula: see text] were used. Quantitative polarized light microscopy (qPLM) methods were developed to account for the relatively high cell density of immature articular cartilage and used with a novel algorithm that constructs a 3D distribution function from 2D qPLM data. For specimens untreated and cultured in vitro, most model parameters were specified from qPLM analysis and biochemical assay results; consequently, [Formula: see text] was predicted using an optimization to measured mechanical properties in uniaxial tension and unconfined compression. Analysis of qPLM data revealed a highly anisotropic fiber distribution, with principal fiber orientation parallel to the surface layer. For untreated samples, predicted [Formula: see text] values were 175 and 422 MPa for superficial (S) and middle (M) zone layers, respectively. TGF-[Formula: see text]1 treatment was predicted to increase and decrease [Formula: see text] values for the S and M layers to 281 and 309 MPa, respectively. IGF-1 treatment was predicted to decrease [Formula: see text] values for the S and M layers to 22 and 26 MPa, respectively. A novel finding was that distinct native depth-dependent fiber modulus properties were modulated to nearly homogeneous values by TGF-[Formula: see text]1 and IGF-1 treatments, with modulated values strongly dependent on treatment.
Michael E Stender; Christopher B Raub; Kevin A Yamauchi; Reza Shirazi; Pasquale Vena; Robert L Sah; Scott J Hazelwood; Stephen M Klisch
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-12-25
Journal Detail:
Title:  Biomechanics and modeling in mechanobiology     Volume:  12     ISSN:  1617-7940     ISO Abbreviation:  Biomech Model Mechanobiol     Publication Date:  2013 Nov 
Date Detail:
Created Date:  2013-10-18     Completed Date:  2014-05-14     Revised Date:  2014-11-04    
Medline Journal Info:
Nlm Unique ID:  101135325     Medline TA:  Biomech Model Mechanobiol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1073-88     Citation Subset:  IM; S    
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MeSH Terms
Biomechanical Phenomena / drug effects
Cartilage, Articular / drug effects,  physiology*
Elastic Modulus / drug effects*
Fibrillar Collagens / metabolism*
Glycosaminoglycans / metabolism
Insulin-Like Growth Factor I / pharmacology*
Microscopy, Polarization / methods*
Models, Biological*
Transforming Growth Factor beta1 / pharmacology*
Grant Support
Reg. No./Substance:
0/Fibrillar Collagens; 0/Glycosaminoglycans; 0/Transforming Growth Factor beta1; 67763-96-6/Insulin-Like Growth Factor I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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