| Integrating molecular detection and response to create self-signalling antibodies. | |
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MedLine Citation:
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PMID: 15474456 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Monoclonal antibodies are reproducible, specific, and cost-effective molecular probes; use outside the laboratory is, however, restricted by technical limitations. Addressing these constraints, the first self-signalling antibodies are now described, where specific antigen binding causes release of bound reporter from bispecific antibodies (BsAb) to generate a detectable signal. The report examines the concept that two different antibody binding sites in close proximity can promote interaction between molecules recognised by these sites, generating a signal by molecular crowding. Signal strength is found to increase with increasing homogeneity for a BsAb reactive with multimeric surfactant antigen; signal response is linear for a BsAb reactive with univalent small analyte deoxypyridinoline. Self-signalling is consistent with intramolecular steric hindrance. This is the first report detailing integration of two different functions, molecular detection and signal response, into BsAbs and with detection of large and small analytes, has generic application to antibody-based systems. |
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Authors:
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Beverley J Randle; Simon J Scoltock; Deborah K Scott |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 324 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2004 Nov |
Date Detail:
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Created Date: 2004-10-11 Completed Date: 2004-12-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 504-10 Citation Subset: IM |
Affiliation:
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Antibody Research Laboratory, Division of Obstetrics, Department of Clinical Sciences at South Bristol, St. Michael's Hospital, University of Bristol, Bristol BS2 8EG, United Kingdom. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Allergy and Immunology* Amino Acids / chemistry Antibodies, Bispecific / chemistry* Antibodies, Monoclonal / chemistry* Antigens / chemistry Apoproteins / chemistry Binding Sites, Antibody Chromatography Chromatography, High Pressure Liquid Dose-Response Relationship, Drug Genes, Reporter Immunoassay / methods Molecular Weight Pulmonary Surfactant-Associated Protein A / chemistry Signal Transduction Surface-Active Agents / chemistry beta-Galactosidase / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Antibodies, Bispecific; 0/Antibodies, Monoclonal; 0/Antigens; 0/Apoproteins; 0/Binding Sites, Antibody; 0/Pulmonary Surfactant-Associated Protein A; 0/Surface-Active Agents; 90032-33-0/deoxypyridinoline; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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