Document Detail


Integrating carbon nanotubes and lipid bilayer for biosensing.
MedLine Citation:
PMID:  20047826     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Membrane proteins, which are the target of most drugs, are implicated in many critical cellular functions such as signal transduction, bioelectricity, exocytosis and endocytosis. Therefore, developing techniques to investigate the functions of membrane proteins is obviously important. Here, we have developed a novel system by integrating artificial lipid bilayer (biomimetic membrane) with single-walled carbon nanotube networks (SWNT-net) based field-effect transistor (FET), and demonstrated that such hybrid nanoelectronic biosensors can specifically and electronically detect the presence and dynamic activities of ionophores (specifically, gramicidin and calcimycin) in their native lipid environment. This technique can potentially be used to examine other membrane proteins (e.g. ligand-gated ion channels, receptors, membrane insertion toxins, and antibacterial peptides) for the purposes of biosensing, fundamental studies, or high throughput drug screening.
Authors:
Yinxi Huang; Preeti Vikas Palkar; Lain-Jong Li; Hua Zhang; Peng Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-12-23
Journal Detail:
Title:  Biosensors & bioelectronics     Volume:  25     ISSN:  1873-4235     ISO Abbreviation:  Biosens Bioelectron     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-02-17     Completed Date:  2010-05-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9001289     Medline TA:  Biosens Bioelectron     Country:  England    
Other Details:
Languages:  eng     Pagination:  1834-7     Citation Subset:  IM    
Copyright Information:
(c) 2009 Elsevier B.V. All rights reserved.
Affiliation:
School of Chemical and Biomedical Engineering, Bioengineering Division, Nanyang Technological University, 70 Nanyang Drive, Block N1.3, Singapore 637457, Singapore.
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MeSH Terms
Descriptor/Qualifier:
Biomimetic Materials / chemistry*
Biosensing Techniques / methods*
Conductometry / methods*
Lipid Bilayers / chemistry*
Membrane Proteins / chemistry*
Nanotechnology / methods*
Nanotubes, Carbon / chemistry*
Systems Integration
Chemical
Reg. No./Substance:
0/Lipid Bilayers; 0/Membrane Proteins; 0/Nanotubes, Carbon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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