Document Detail

Integrated view of genome structure and sequence of a single DNA molecule in a nanofluidic device.
MedLine Citation:
PMID:  23479649     Owner:  NLM     Status:  MEDLINE    
We show how a bird's-eye view of genomic structure can be obtained at ∼1-kb resolution from long (∼2 Mb) DNA molecules extracted from whole chromosomes in a nanofluidic laboratory-on-a-chip. We use an improved single-molecule denaturation mapping approach to detect repetitive elements and known as well as unique structural variation. Following its mapping, a molecule of interest was rescued from the chip; amplified and localized to a chromosome by FISH; and interrogated down to 1-bp resolution with a commercial sequencer, thereby reconciling haplotype-phased chromosome substructure with sequence.
Rodolphe Marie; Jonas N Pedersen; David L V Bauer; Kristian H Rasmussen; Mohammed Yusuf; Emanuela Volpi; Henrik Flyvbjerg; Anders Kristensen; Kalim U Mir
Related Documents :
23237939 - Inhibition of homologous recombination by hyperthermia shunts early double strand break...
22487099 - Chromosome rearrangements during domestication of cucumber as revealed from high-densit...
23498939 - X-inactivation, imprinting, and long noncoding rnas in health and disease.
23335129 - Neurocognitive variance and neurological underpinnings of the x and y chromosomal varia...
24413689 - Studies of dotted, a regulatory element in maize : iii. transpositions of dotted and it...
22714999 - Targeted therapy: the new lease on life for acute promyelocytic leukemia, and beyond.
2440709 - Clonal nature of philadelphia chromosome-positive and -negative chronic myelogenous leu...
9549059 - Collinearity between a 30-centimorgan segment of arabidopsis thaliana chromosome 4 and ...
10557299 - Participation of bir1p, a member of the inhibitor of apoptosis family, in yeast chromos...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2013-03-11
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  110     ISSN:  1091-6490     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-03-27     Completed Date:  2013-05-23     Revised Date:  2013-07-11    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  United States    
Other Details:
Languages:  eng     Pagination:  4893-8     Citation Subset:  IM    
Department of Micro- and Nanotechnology, Technical University of Denmark, DK-2800 Kongens Lyngby, Denmark.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Chromosome Mapping* / instrumentation,  methods
Chromosomes, Human* / chemistry,  genetics
DNA* / chemistry,  genetics
Genome, Human*
In Situ Hybridization, Fluorescence / instrumentation,  methods
Microfluidic Analytical Techniques* / instrumentation,  methods
Grant Support
090532/Z/09/Z//Wellcome Trust
Reg. No./Substance:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Governance regime and location influence avoided deforestation success of protected areas in the Bra...
Next Document:  Portraying the unique contribution of the default mode network to internally driven mnemonic process...