| Integrated single-cell analysis shows Pichia pastoris secretes protein stochastically. | |
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MedLine Citation:
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PMID: 20148400 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The production of heterologous proteins by secretion from cellular hosts is an important determinant for the cost of biotherapeutics. A single-cell analytical method called microengraving was used to examine the heterogeneity in secretion by the methylotrophic yeast Pichia pastoris. We show that constitutive secretion of a human Fc fragment by P. pastoris is not cell-cycle dependent, but rather fluctuates between states of high and low productivity in a stochastic manner. |
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Authors:
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Kerry Routenberg Love; Vasiliki Panagiotou; Bo Jiang; Terrance A Stadheim; J Christopher Love |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biotechnology and bioengineering Volume: 106 ISSN: 1097-0290 ISO Abbreviation: Biotechnol. Bioeng. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-04-26 Completed Date: 2010-07-27 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7502021 Medline TA: Biotechnol Bioeng Country: United States |
Other Details:
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Languages: eng Pagination: 319-25 Citation Subset: IM |
Affiliation:
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Department of Chemical Engineering, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, Massachusetts 02139, USA. george.miesegaes@fda.hhs.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Culture Techniques
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instrumentation* Computer Simulation Equipment Design Gene Expression Profiling / instrumentation* Models, Biological* Pichia / physiology* Protein Array Analysis / instrumentation* Recombinant Proteins / metabolism* Stochastic Processes Systems Integration |
| Chemical | |
Reg. No./Substance:
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0/Recombinant Proteins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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