| Integrated multimodal endomicroscopy platform for simultaneous en face optical coherence and two-photon fluorescence imaging. | |
| | |
MedLine Citation:
|
PMID: 22297353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
We report an all-fiber-optic scanning, multimodal endomicroscope capable of simultaneous optical coherence tomography (OCT) and two-photon fluorescence (TPF) imaging. Both imaging modalities share the same miniature fiber-optic scanning endomicroscope, which consists of a double-clad fiber with a core operating in single mode at both the OCT (1310 nm) and two-photon excitation (1550 nm) wavelengths, a piezoelectric two-dimensional fiber-optic beam scanner, and a miniature aspherical compound lens suitable for simultaneous acquisition of en face OCT and TPF images. A fiber-optic wavelength division multiplexer was employed in the integrated platform to combine the low coherence OCT light source and the femtosecond two-photon excitation laser into the same optical path. Preliminary imaging results of cell cultures and mouse tissue ex vivo demonstrate the feasibility of simultaneous real-time OCT and TPF imaging in a scanning endomicroscopy setting for the first time. |
| | |
Authors:
|
Jiefeng Xi; Yongping Chen; Yuying Zhang; Kartikeya Murari; Ming-Jun Li; Xingde Li |
Related Documents
:
|
22611663 - Perceptual filling-in of negative coloured afterimages. 22874883 - Adaptive thresholding algorithm based on sar images and wind data to segment oil spills... 15741013 - Post colon surgery complications: imaging findings. |
Publication Detail:
|
Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Optics letters Volume: 37 ISSN: 1539-4794 ISO Abbreviation: Opt Lett Publication Date: 2012 Feb |
Date Detail:
|
Created Date: 2012-02-02 Completed Date: 2012-05-16 Revised Date: 2012-06-26 |
Medline Journal Info:
|
Nlm Unique ID: 7708433 Medline TA: Opt Lett Country: United States |
Other Details:
|
Languages: eng Pagination: 362-4 Citation Subset: IM |
Affiliation:
|
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, Maryland 21210, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adipose Tissue
/
metabolism Animals Cell Line, Tumor Humans Mice Microscopy, Fluorescence / methods* Photons* Time Factors Tomography, Optical Coherence / methods* |
| Grant Support | |
ID/Acronym/Agency:
|
R01 CA120480/CA/NCI NIH HHS; R01 CA120480/CA/NCI NIH HHS; R01 CA153023/CA/NCI NIH HHS; R01 CA153023/CA/NCI NIH HHS; R01 EB007636/EB/NIBIB NIH HHS |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Enhance the resolution of photonic crystal negative refraction imaging by metal grating.
Next Document: Ultracompact polarization converter with a dual subwavelength trench built in a silicon-on-insulator...