Document Detail


Integrated multimodal endomicroscopy platform for simultaneous en face optical coherence and two-photon fluorescence imaging.
MedLine Citation:
PMID:  22297353     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We report an all-fiber-optic scanning, multimodal endomicroscope capable of simultaneous optical coherence tomography (OCT) and two-photon fluorescence (TPF) imaging. Both imaging modalities share the same miniature fiber-optic scanning endomicroscope, which consists of a double-clad fiber with a core operating in single mode at both the OCT (1310 nm) and two-photon excitation (1550 nm) wavelengths, a piezoelectric two-dimensional fiber-optic beam scanner, and a miniature aspherical compound lens suitable for simultaneous acquisition of en face OCT and TPF images. A fiber-optic wavelength division multiplexer was employed in the integrated platform to combine the low coherence OCT light source and the femtosecond two-photon excitation laser into the same optical path. Preliminary imaging results of cell cultures and mouse tissue ex vivo demonstrate the feasibility of simultaneous real-time OCT and TPF imaging in a scanning endomicroscopy setting for the first time.
Authors:
Jiefeng Xi; Yongping Chen; Yuying Zhang; Kartikeya Murari; Ming-Jun Li; Xingde Li
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Optics letters     Volume:  37     ISSN:  1539-4794     ISO Abbreviation:  Opt Lett     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-02-02     Completed Date:  2012-05-16     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  7708433     Medline TA:  Opt Lett     Country:  United States    
Other Details:
Languages:  eng     Pagination:  362-4     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / metabolism
Animals
Cell Line, Tumor
Humans
Mice
Microscopy, Fluorescence / methods*
Photons*
Time Factors
Tomography, Optical Coherence / methods*
Grant Support
ID/Acronym/Agency:
R01 CA120480/CA/NCI NIH HHS; R01 CA120480/CA/NCI NIH HHS; R01 CA153023/CA/NCI NIH HHS; R01 CA153023/CA/NCI NIH HHS; R01 EB007636/EB/NIBIB NIH HHS; R01 EB007636/EB/NIBIB NIH HHS
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