Document Detail


Integrated hepatic transcriptome and proteome analysis of mice with high-fat diet-induced nonalcoholic fatty liver disease.
MedLine Citation:
PMID:  20303728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nonalcoholic fatty liver disease (NAFLD) is the most common form of liver disease in the US and refers to a wide spectrum of liver damage, including simple steatosis, steatohepatitis, fibrosis and cirrhosis. The goal of the present study was to achieve a more detailed understanding of the molecular changes in response to high fat-induced liver steatosis through the identification of a differentially expressed liver transcriptome and proteome. Male C57/BL6 mice fed a high-fat lard diet for 8 weeks developed visceral obesity and hepatic steatosis characterized by significantly increased liver and plasma free fatty acid and triglyceride levels and plasma alanine aminotransferase activities. Transcriptome analysis demonstrated that, compared to the control diet (CD), high-fat diet changed the expression of 309 genes (132 up- and 177 down-regulated; by a twofold change and more, P<.05). Multiple genes encoding proteins involved in lipogenesis were down-regulated, whereas genes involved in fatty acid oxidation were up-regulated. Proteomic analysis revealed 12 proteins which were differentially expressed. Of these, glutathione S-transferases mu1 and pi1 and selenium-binding protein 2 were decreased at both the gene and protein levels. This is the first study to perform a parallel transcriptomic and proteomic analysis of diet-induced hepatic steatosis. Several key pathways involving xenobiotic and lipid metabolism, the inflammatory response and cell-cycle control were identified. These pathways provide targets for future mechanistic and therapeutic studies as related to the development and prevention of NAFLD.
Authors:
Irina A Kirpich; Leila N Gobejishvili; Marjorie Bon Homme; Sabine Waigel; Matt Cave; Gavin Arteel; Shirish S Barve; Craig J McClain; Ion V Deaciuc
Related Documents :
20096718 - Effects of selective breeding for increased wheel-running behavior on circadian timing ...
24560278 - The beneficial effects of rosuvastatin are independent of zinc supplementation in patie...
18292748 - Sequential responses to high-fat and high-calorie feeding in an obese mouse model.
18063868 - Effect of aging on fatty streak formation in a diet-induced mouse model of atherosclero...
3724048 - Nutritional deficiency anemias in nonhuman primates.
18487668 - Influence of anionic salts on bone metabolism in periparturient dairy goats and sheep.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Validation Studies     Date:  2010-03-20
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  22     ISSN:  1873-4847     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2010-12-14     Completed Date:  2011-03-24     Revised Date:  2014-09-09    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  38-45     Citation Subset:  IM    
Copyright Information:
Copyright © 2011. Published by Elsevier Inc.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Dietary Fats / adverse effects
Fatty Acids / metabolism
Fatty Liver / metabolism*,  pathology
Gene Expression Profiling
Gene Expression Regulation*
Glutathione S-Transferase pi / chemistry,  genetics,  metabolism
Glutathione Transferase / chemistry,  genetics,  metabolism
Lipogenesis
Liver / metabolism*,  pathology
Male
Mice
Mice, Inbred C57BL
Oligonucleotide Array Sequence Analysis
Proteome / chemistry,  genetics,  metabolism*
RNA, Messenger / metabolism
Selenium-Binding Proteins / chemistry,  genetics,  metabolism
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry
Two-Dimensional Difference Gel Electrophoresis
Grant Support
ID/Acronym/Agency:
K23 AA018399/AA/NIAAA NIH HHS; P01 AA017103/AA/NIAAA NIH HHS; P01AA017103/AA/NIAAA NIH HHS; P20 RR16481/RR/NCRR NIH HHS; P20RR018733/RR/NCRR NIH HHS; P30 ESO14443//PHS HHS; R01 AA018016/AA/NIAAA NIH HHS; R01 AA018869/AA/NIAAA NIH HHS; R01 DK071765/DK/NIDDK NIH HHS; R01 ES021375/ES/NIEHS NIH HHS; R01AA015970/AA/NIAAA NIH HHS; R01AA018016/AA/NIAAA NIH HHS; R01DK071765/DK/NIDDK NIH HHS; R21AA015611/AA/NIAAA NIH HHS; R37 AA010762/AA/NIAAA NIH HHS; R37AA010762/AA/NIAAA NIH HHS
Chemical
Reg. No./Substance:
0/Dietary Fats; 0/Fatty Acids; 0/Proteome; 0/RNA, Messenger; 0/Selenbp2 protein, mouse; 0/Selenium-Binding Proteins; 61789-99-9/lard; EC 2.5.1.18/Glutathione S-Transferase pi; EC 2.5.1.18/Glutathione Transferase; EC 2.5.1.18/Gstp1 protein, mouse; EC 2.5.1.18/glutathione S-transferase M1
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Involvement of nucleophosmin/B23 in the cellular response to curcumin.
Next Document:  Emulsified lipids increase endotoxemia: possible role in early postprandial low-grade inflammation.