| Integrated electrical concentration and lysis of cells in a microfluidic chip. | |
| | |
MedLine Citation:
|
PMID: 20981237 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Lysing cells is an important step in the analysis of intracellular contents. Concentrating cells is often required in order to acquire adequate cells for lysis. This work presents an integrated concentration and lysis of mammalian cells in a constriction microchannel using dc-biased ac electric fields. By adjusting the dc component, the electrokinetic cell motion can be precisely controlled, leading to an easy switch between concentration and lysis of red blood cells in the channel constriction. These two operations are also used in conjunction to demonstrate a continuous concentration and separation of leukemia cells from red blood cells in the same microchannel. The observed cell behaviors agree reasonably with the simulation results. |
| | |
Authors:
|
Christopher Church; Junjie Zhu; Guohui Huang; Tzuen-Rong Tzeng; Xiangchun Xuan |
Related Documents
:
|
20800467 - Recording electric potentials from single adherent cells with 3d microelectrode arrays ... 11438707 - Tat peptide on the surface of liposomes affords their efficient intracellular delivery ... 16718407 - Dielectrophoresis tweezers for single cell manipulation. 20221517 - Large-pore sized mesoporous carbon electrocatalyst for efficient dye-sensitized solar c... 14759767 - Cell cycle and behaviour in euplotes crassus: an attempt at describing the biological r... 6798197 - Passive potassium transport in low potassium sheep red cells: dependence upon cell volu... |
Publication Detail:
|
Type: Journal Article Date: 2010-10-01 |
Journal Detail:
|
Title: Biomicrofluidics Volume: 4 ISSN: 1932-1058 ISO Abbreviation: Biomicrofluidics Publication Date: 2010 |
Date Detail:
|
Created Date: 2010-10-28 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 101293825 Medline TA: Biomicrofluidics Country: United States |
Other Details:
|
Languages: eng Pagination: 44101 Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Genome-wide linkage and association scans for quantitative trait Loci of serum lactate dehydrogenase...
Next Document: Thermal mixing of two miscible fluids in a T-shaped microchannel.