Document Detail


Integrated bioinformatic and phenotypic analysis of RpoN-dependent traits in the plant growth-promoting bacterium Pseudomonas fluorescens SBW25.
MedLine Citation:
PMID:  17991033     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The alternative sigma factor RpoN is a key regulator in the acclimation of Pseudomonas to complex natural environments. In this study we show that RpoN is required for efficient colonization of sugar beet seedlings by the plant growth-promoting bacterium Pseudomonas fluorescens SBW25, and use phenotypic and bioinformatic approaches to profile the RpoN-dependent traits and genes of P. fluorescens SBW25. RpoN is required for flagellar biosynthesis and for assimilation of a wide variety of nutrient sources including inorganic nitrogen, amino acids, sugar alcohols and dicarboxylic acids. Chemosensitivity assays indicate that RpoN-regulated genes contribute to acid tolerance and resistance to some antibiotics, including tetracyclines and aminoglycosides. Gain of function changes associated with loss of RpoN included increased tolerance to hydroxyurea and Guanazole. Bioinformatic predictions of RpoN-regulated genes show a close correspondence with phenotypic analyses of RpoN-regulated traits and suggest novel functions for RpoN in P. fluorescens, including regulation of poly(A) polymerase. The reduced plant colonization ability observed for an rpoN mutant of P. fluorescens is therefore likely to be due to defects in multiple traits including nutrient assimilation, protein secretion and stress tolerance.
Authors:
Jacob Jones; David J Studholme; Christopher G Knight; Gail M Preston
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Environmental microbiology     Volume:  9     ISSN:  1462-2912     ISO Abbreviation:  Environ. Microbiol.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-09     Completed Date:  2008-01-08     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100883692     Medline TA:  Environ Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  3046-64     Citation Subset:  IM    
Affiliation:
Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX13RB, UK.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Proteins / genetics,  metabolism
Base Sequence
Beta vulgaris / growth & development,  microbiology*
Binding Sites
Computational Biology / methods*
Gene Deletion
Gene Expression Regulation, Bacterial*
Markov Chains
Molecular Sequence Data
Oligonucleotide Array Sequence Analysis / methods
Phenotype
Plant Roots / microbiology
Pseudomonas fluorescens / classification*,  genetics,  metabolism
RNA Polymerase Sigma 54 / genetics,  metabolism*
Soil Microbiology
Chemical
Reg. No./Substance:
0/Bacterial Proteins; EC 2.7.7.6/RNA Polymerase Sigma 54

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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