| Intact and permeabilized cells of the yeast Hansenula polymorpha as bioselective elements for amperometric assay of formaldehyde. | |
| | |
MedLine Citation:
|
PMID: 19071397 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
Intact and permeabilized yeast cells were tested as the biorecognition elements for amperometric assay of formaldehyde (FA). For this aim, the mutant C-105 (gcr1 catX) of the methylotrophic yeast Hansenula polymorpha with a high activity of AOX was chosen. Different approaches were used for monitoring FA-dependent cell response including analysis of their oxygen consumption rate by the use of a Clark electrode, as well as assay of oxidation of redox mediator at a screen-printed platinum electrode covered by cells entrapped in Ca-alginate gel. It was shown that oxygen consumption rate of permeabilized cells reached its saturation at 4mM of FA (23 degrees C). The detection limit was found to be 0.27mM. In the presence of redox mediator 2,6-dichlorophenolindophenol (DCIP), the screen-printed platinum band electrode covered by permeabilized cells did not show any current output to FA. In contrast, well-pronounced amperometric response to FA was observed in the case of intact yeast cells in the presence of DCIP. It was shown that current output reached its maximum at 7mM concentration of FA. The detection limit was found to be 0.74mM. Obviously, it is necessary to perform a directed genetic engineering of the yeast cells to improve their bioanalytical characteristics in the corresponding biosensors. |
| | |
Authors:
|
Maria Khlupova; Boris Kuznetsov; Olha Demkiv; Mykhailo Gonchar; Elisabeth Csöregi; Sergey Shleev |
Related Documents
:
|
20883447 - Bactenecin 7 peptide fragment as a tool for intracellular delivery of a phosphorescent ... 3252027 - Comparison of the toxicity of the radiocontrast agents, iopamidol and diatrizoate, to r... 7971747 - A comparison between melanotic and amelanotic retinal pigment epithelial cells in vitro... 3178397 - Reversible conversion of coenzyme f420 to the 8-oh-amp and 8-oh-gmp esters, f390-a and ... 3743867 - Communication between primary endoderm and mesoderm for erythroblast differentiation in... 17182797 - Activation of caspase-8 contributes to 3,3'-diindolylmethane-induced apoptosis in colon... |
Publication Detail:
|
Type: Journal Article Date: 2006-07-10 |
Journal Detail:
|
Title: Talanta Volume: 71 ISSN: 1873-3573 ISO Abbreviation: Talanta Publication Date: 2007 Feb |
Date Detail:
|
Created Date: 2008-12-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 2984816R Medline TA: Talanta Country: England |
Other Details:
|
Languages: eng Pagination: 934-40 Citation Subset: - |
Affiliation:
|
Laboratory of Chemical Enzymology, A.N. Bach Institute of Biochemistry, 119071 Moscow, Russia. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Development of a group-specific immunoassay for sulfonamides. Application to bee honey analysis.
Next Document: Sequential injection analyzer for glycerol monitoring in yeast cultivation medium.