Document Detail


Insulin stimulates inositol incorporation in hippocampus of lean but not obese Zucker rats.
MedLine Citation:
PMID:  2159162     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have previously reported that intraventricular insulin is ineffective in decreasing the body weight of obese (fa/fa) Zucker rats. In the present study, we report that insulin at concentrations of 1 and 5 nM significantly stimulates incorporation of 3H-myoinositol into inositol phosphates (29 +/- 5% and 20 +/- 6% stimulation over control levels) and membrane inositol lipids (29 +/- 4% and 20 +/- 6% stimulation over control levels) within hippocampal slices from lean (Fa/Fa) Zucker rats (n = 3 preparations). Smaller but significant stimulations were observed in the Fa/fa Zucker rat (n = 5) hippocampus [10 +/- 3 and 13 +/- 4% stimulation over control levels (inositol phosphates) and 10 +/- 4 and 14 +/- 3% stimulation over control levels (inositol lipids) with 1 and 5 nM insulin]. Insulin had no effect on 3H-inositol incorporation into hippocampal slices from obese (fa/fa) Zucker rats (n = 5). No difference in insulin binding to hippocampal membranes was observed among the three genotypes. These findings confirm the behavioral observation of insulin insensitivity within the fa/fa Zucker CNS and suggest that this insensitivity may be gene-dose-related.
Authors:
D Figlewicz; P Szot; M R Greenwood
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Physiology & behavior     Volume:  47     ISSN:  0031-9384     ISO Abbreviation:  Physiol. Behav.     Publication Date:  1990 Feb 
Date Detail:
Created Date:  1990-06-05     Completed Date:  1990-06-05     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0151504     Medline TA:  Physiol Behav     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  325-30     Citation Subset:  IM    
Affiliation:
Department of Psychology, University of Washington, Seattle 98195.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Glucose / metabolism
Female
Hippocampus / drug effects*
Inositol / metabolism*
Inositol Phosphates / metabolism
Insulin / metabolism,  pharmacology*
Membrane Lipids / metabolism*
Obesity / metabolism*
Phosphatidylinositols / metabolism
Protein Kinase C / metabolism
Rats
Rats, Zucker
Receptor, Insulin / drug effects
Synaptic Membranes / metabolism*
Grant Support
ID/Acronym/Agency:
AM 26687/AM/NIADDK NIH HHS; DK40963-01/DK/NIDDK NIH HHS; HD 12637/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Blood Glucose; 0/Inositol Phosphates; 0/Membrane Lipids; 0/Phosphatidylinositols; 11061-68-0/Insulin; 6917-35-7/Inositol; EC 2.7.10.1/Receptor, Insulin; EC 2.7.11.13/Protein Kinase C

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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