| Insulin-stimulated phosphoinositide metabolism in isolated fat cells. | |
| | |
MedLine Citation:
|
PMID: 2993299 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Treatment of isolated fat cells with insulin produced increases of up to 4.8-fold in the incorporation of [3H]inositol into phosphatidylinositol. This effect of insulin was both time- and dose-dependent with half-maximal stimulation at 30 microunits/ml of insulin. Insulin increased the labeling of phosphatidylinositol and phosphatidylinositol 4,5-bisphosphate but not phosphatidylinositol 4-monophosphate in cells which had been preincubated with [3H]inositol for 90 min. Incubation of the cells in a Ca2+-free buffer increased the basal level of phosphatidylinositol labeling and enhanced the effect of insulin. Glucagon and isoprenaline, both of which stimulate lipolysis, had no effect on phosphatidylinositol labeling but did potentiate insulin-stimulated incorporation of [3H]inositol into phosphatidylinositol. Phosphoinositide breakdown was measured by the accumulation of inositol phosphates. Insulin did not increase the level of the inositol phosphates at all concentrations of the hormone tested. By comparison, phenylephrine and vasopressin were able to stimulate phosphoinositide breakdown. Pretreatment of the cells with insulin enhanced the effect of phenylephrine on inositol phosphates' accumulation, suggesting that insulin may potentiate phenylephrine-mediated phosphoinositide turnover. From these data we conclude that insulin stimulates the de novo synthesis of phosphatidylinositol and phosphatidylinositol 4,5-biphosphate, but has no effect on phosphoinositide breakdown. |
| | |
Authors:
|
S R Pennington; B R Martin |
Related Documents
:
|
18768479 - Reciprocal control of pyruvate dehydrogenase kinase and phosphatase by inositol phospho... 1659989 - Phenylarsine oxide stimulates a cytosolic tyrosine kinase activity and glucose transpor... 18078859 - Statin modulates insulin signaling and insulin resistance in liver and muscle of rats f... 12133089 - Clinical results with a new acoustic device to identify the epidural space. 4393739 - Posttranscriptional control of tyrosine aminotransferase synthesis by insulin. 15561949 - Insulin stimulates and diabetes inhibits o-linked n-acetylglucosamine transferase and o... 19933989 - Depression and advanced complications of diabetes: a prospective cohort study. 10439629 - Regulation of insulin-like growth factor-i in starvation and injury. 2498609 - Effect of insulin on plasma norepinephrine and 3,4-dihydroxyphenylalanine in obese men. |
Publication Detail:
|
Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: The Journal of biological chemistry Volume: 260 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1985 Sep |
Date Detail:
|
Created Date: 1985-10-11 Completed Date: 1985-10-11 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 11039-45 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Adipose Tissue
/
drug effects,
metabolism* Animals Calcium / pharmacology Egtazic Acid / pharmacology Inositol / metabolism Insulin / pharmacology* Kinetics Male Phosphatidylinositols / metabolism* Phospholipids / biosynthesis Rats Rats, Inbred Strains Tritium |
| Chemical | |
Reg. No./Substance:
|
0/Phosphatidylinositols; 0/Phospholipids; 10028-17-8/Tritium; 11061-68-0/Insulin; 67-42-5/Egtazic Acid; 6917-35-7/Inositol; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Regulation of calcium transport by protein phosphatase activity associated with cardiac sarcoplasmic...
Next Document: Bovine brain calmodulin-dependent protein phosphatase. Regulation of subunit A activity by calmoduli...