Document Detail

Insulin secretion and intracellular Ca2+ rises in monolayer cultures of neonatal rat beta-cells.
MedLine Citation:
PMID:  7718409     Owner:  NLM     Status:  MEDLINE    
Glucose-induced insulin release, glucose-induced rises in intracellular free Ca2+ concentration ([Ca2+]i), and voltage-dependent Ca2+ channel activity were assessed in monolayer cultures of beta-cells from 3-5-day-old rats. The glucose-stimulated insulin secretory responses and [Ca2+]i rises were like those in adult rat beta-cells rather than fetal rat beta-cells. Voltage-dependent Ca2+ channel antagonists decreased glucose-induced insulin secretion, aborted the [Ca2+]i rise and, like deprivation of extracellular Ca2+, prevented the glucose-induced rise in [Ca2+]i when added before the glucose challenge. The presence of nifedipine-sensitive, voltage-dependent Ca2+ channels was demonstrated directly by measuring Ca2+ currents using the whole-cell configuration of the patch-clamp technique and indirectly by measuring [Ca2+]i after membrane depolarization by 45 mM K+ or 200 microM tolbutamide. Thus, in cultured beta-cells of 3-5-day-old rats the coupling of glucose stimulation to Ca2+ influx is essentially mature, in contrast to what has been reported for fetal or very early neonatal cells.
M A Black; G A Mealing; J F Whitfield; J L Schwartz; N Bégin-Heick
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular signalling     Volume:  6     ISSN:  0898-6568     ISO Abbreviation:  Cell. Signal.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-05-25     Completed Date:  1995-05-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8904683     Medline TA:  Cell Signal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  897-904     Citation Subset:  IM    
Department of Biochemistry, University of Ottawa, Ontario, Canada.
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MeSH Terms
Age Factors
Calcium / metabolism*
Calcium Channels / physiology
Cells, Cultured
Glucose / physiology
Insulin / secretion*
Islets of Langerhans / drug effects,  metabolism*,  physiology
Nifedipine / pharmacology
Patch-Clamp Techniques
Potassium / pharmacology
Rats, Sprague-Dawley
Tolbutamide / pharmacology
Reg. No./Substance:
0/Calcium Channels; 11061-68-0/Insulin; 21829-25-4/Nifedipine; 50-99-7/Glucose; 64-77-7/Tolbutamide; 7440-09-7/Potassium; 7440-70-2/Calcium

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