Document Detail


Insulin secretion and intracellular Ca2+ rises in monolayer cultures of neonatal rat beta-cells.
MedLine Citation:
PMID:  7718409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Glucose-induced insulin release, glucose-induced rises in intracellular free Ca2+ concentration ([Ca2+]i), and voltage-dependent Ca2+ channel activity were assessed in monolayer cultures of beta-cells from 3-5-day-old rats. The glucose-stimulated insulin secretory responses and [Ca2+]i rises were like those in adult rat beta-cells rather than fetal rat beta-cells. Voltage-dependent Ca2+ channel antagonists decreased glucose-induced insulin secretion, aborted the [Ca2+]i rise and, like deprivation of extracellular Ca2+, prevented the glucose-induced rise in [Ca2+]i when added before the glucose challenge. The presence of nifedipine-sensitive, voltage-dependent Ca2+ channels was demonstrated directly by measuring Ca2+ currents using the whole-cell configuration of the patch-clamp technique and indirectly by measuring [Ca2+]i after membrane depolarization by 45 mM K+ or 200 microM tolbutamide. Thus, in cultured beta-cells of 3-5-day-old rats the coupling of glucose stimulation to Ca2+ influx is essentially mature, in contrast to what has been reported for fetal or very early neonatal cells.
Authors:
M A Black; G A Mealing; J F Whitfield; J L Schwartz; N Bégin-Heick
Related Documents :
12196469 - Glucose-sensing in glucagon-like peptide-1-secreting cells.
9051699 - Dissociation between the potency and reversibility of the insulinotropic action of two ...
109129 - Insulin action in isolated fat cells. i. effects of divalent cations on the stimulation...
9366569 - Pulsatile insulin release from pancreatic islets with nonoscillatory elevation of cytop...
2505929 - Insulin release from pancreatic islets of fetal rats mediated by leucine b-bch, tolbuta...
6814899 - Lipoxygenation of arachidonic acid: a pivotal step in stimulus-secretion coupling in th...
18805479 - Constriction of pulmonary artery by peroxide: role of ca2+ release and pkc.
3947349 - Calcium transport by ionophorous peptides in dog and human lymphocytes detected by quin...
3265649 - Strychnine decreases the voltage-dependent ca2+ current of both aplysia and frog gangli...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cellular signalling     Volume:  6     ISSN:  0898-6568     ISO Abbreviation:  Cell. Signal.     Publication Date:  1994 Nov 
Date Detail:
Created Date:  1995-05-25     Completed Date:  1995-05-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8904683     Medline TA:  Cell Signal     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  897-904     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, University of Ottawa, Ontario, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Age Factors
Animals
Calcium / metabolism*
Calcium Channels / physiology
Cells, Cultured
Female
Glucose / physiology
Insulin / secretion*
Islets of Langerhans / drug effects,  metabolism*,  physiology
Male
Nifedipine / pharmacology
Patch-Clamp Techniques
Potassium / pharmacology
Rats
Rats, Sprague-Dawley
Tolbutamide / pharmacology
Chemical
Reg. No./Substance:
0/Calcium Channels; 11061-68-0/Insulin; 21829-25-4/Nifedipine; 50-99-7/Glucose; 64-77-7/Tolbutamide; 7440-09-7/Potassium; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Calcium uptake and gp80 messenger RNA destabilization follows cAMP receptor down regulation in Dicty...
Next Document:  Mechanisms of ATP-induced Ca2+ signaling in osteoclasts.