Document Detail


Insulin secretion from human beta cells is heterogeneous and dependent on cell-to-cell contacts.
MedLine Citation:
PMID:  18665347     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS/HYPOTHESIS: We assessed the heterogeneity of insulin secretion from human isolated beta cells and its regulation by cell-to-cell contacts. METHODS: Insulin secretion from single and paired cells was assessed by a reverse haemolytic plaque assay. The percentage of plaque-forming cells, the mean plaque area and the total plaque development were evaluated after 1 h of stimulation with different secretagogues. RESULTS: Not all beta cells were surrounded by a haemolytic plaque under all conditions tested. A small fraction of the beta cell population (20%) secreted more than 90% and 70% of total insulin at 2.2 and 22.2 mmol/l glucose, respectively. Plaque-forming cells, mean plaque area and total plaque development were increased at 12.2 and 22.2 compared with 2.2 mmol/l glucose. Insulin secretion of single beta cells was similar at 12.2 and 22.2 mmol/l glucose. Insulin secretion of beta cell pairs was increased compared with that of single beta cells and was higher at 22.2 than at 12.2 mmol/l glucose. Insulin secretion of beta cells in contact with alpha cells was also increased compared with single beta cells, but was similar at 22.2 compared with 12.2 mmol/l glucose. Delta and other non-beta cells did not increase insulin secretion of contacting beta cells compared with that of single beta cells. Differences in insulin secretion between 22.2 and 12.2 mmol/l glucose were observed in murine but not in human islets. CONCLUSIONS/INTERPRETATION: Human beta cells are highly heterogeneous in terms of insulin secretion so that a small fraction of beta cells contributes to the majority of insulin secreted. Homologous and heterologous intercellular contacts have a significant impact on insulin secretion and this could be related to the particular architecture of human islets.
Authors:
A Wojtusciszyn; M Armanet; P Morel; T Berney; D Bosco
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-07-30
Journal Detail:
Title:  Diabetologia     Volume:  51     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-05     Completed Date:  2009-01-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1843-52     Citation Subset:  IM    
Affiliation:
Cell Isolation and Transplantation Center, Department of Surgery, Geneva University Hospitals, 1 rue Michel Servet, Genève-4, Switzerland.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Communication / physiology*
Glucose / secretion
Humans
Immunohistochemistry
Insulin / secretion*
Insulin-Secreting Cells / cytology,  metabolism*
Islets of Langerhans / cytology,  metabolism*
Male
Mice
Chemical
Reg. No./Substance:
11061-68-0/Insulin; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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