Document Detail


Insulin resistance in cancer patients is associated with enhanced tumor necrosis factor-alpha expression in skeletal muscle.
MedLine Citation:
PMID:  9918825     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The roles of tumor necrosis factor (TNF)-alpha and the facilitative glucose transporter (GLUT) 4 on the induction of insulin resistance in peripheral tissues of cancer patients was examined by quantitative competitive PCR on biopsies of abdominal rectal muscle from patients with gastrointestinal cancer. The degree of insulin resistance in these patients was measured by the euglycemic hyperinsulinemic glucose clamp using a high physiologic insulin concentration (100 microU/ml). Quantitative competitive PCR was carried out using DNA competitors constructed by deleting 20-30 bp between the two primer annealing sites. Decreased glucose uptake (M value) in peripheral tissues was accompanied by a significantly increased TNF-alpha mRNA in skeletal muscle (r=0.867, p=0.0025). GLUT4 mRNA, however, was positively correlated with M values (r=0.739, p=0.015). The amounts of mRNAs for TNF-alpha and GLUT4 in skeletal muscle were not correlated. Serum TNF-alpha concentrations remained below the limit of detection. These findings suggest that the insulin resistance in peripheral tissues of cancer patients is in part due to the induction TNF-alpha mRNA and the down regulation of GLUT4 mRNA in peripheral tissues.
Authors:
Y Noguchi; T Yoshikawa; D Marat; C Doi; T Makino; K Fukuzawa; A Tsuburaya; S Satoh; T Ito; S Mitsuse
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  253     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1998 Dec 
Date Detail:
Created Date:  1999-02-09     Completed Date:  1999-02-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  887-92     Citation Subset:  IM    
Affiliation:
First Department of Surgery, Yokohama City University School of Medicine.
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MeSH Terms
Descriptor/Qualifier:
Female
Gastrointestinal Neoplasms / metabolism*
Glucose / metabolism
Glucose Clamp Technique
Glucose Transporter Type 4
Humans
Insulin / pharmacology
Insulin Resistance*
Male
Middle Aged
Monosaccharide Transport Proteins
Muscle Proteins*
Muscle, Skeletal / metabolism*
Tumor Necrosis Factor-alpha / biosynthesis*
Chemical
Reg. No./Substance:
0/Glucose Transporter Type 4; 0/Monosaccharide Transport Proteins; 0/Muscle Proteins; 0/SLC2A4 protein, human; 0/Tumor Necrosis Factor-alpha; 11061-68-0/Insulin; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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