| Insulin releasing and alpha-glucosidase inhibitory activity of ethyl acetate fraction of Acorus calamus in vitro and in vivo. | |
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MedLine Citation:
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PMID: 20051258 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ETHNOPHARMACOLOGICAL RELEVANCE: The radix of Acorus calamus L. (AC) is widely used in the therapy of diabetes in traditional folk medicine of America and Indonesia, and we previously reported the insulin sensitizing activity of the ethyl acetate fraction of AC (ACE). AIM OF THE STUDY: To investigate the insulin releasing and alpha-glucosidase inhibitory activity of ACE in vitro and in vivo. MATERIALS AND METHODS: Insulin releasing and alpha-glucosidase inhibitory effects of different fractions from AC were detected in vitro using HIT-T15 cell line and alpha-glucosidase enzyme. Furthermore, effects of ACE orally on serum glucose were detected in fasted and glucose/amylum challenged normal mice. RESULTS: AC and ACE increased insulin secretion in HIT-T15 cells as gliclazide did. As in vivo results, ACE (400 and 800 mg/kg) significantly decreased fasting serum glucose, and suppressed the increase of blood glucose levels after 2g/kg glucose loading in normal mice. In addition, ACE as a mixed-type inhibitor inhibited alpha-glucosidase activity in vitro with an IC(50) of 0.41 microg/ml, and 100mg/kg of it clearly reduced the increase of blood glucose levels after 5 g/kg amylum loading in normal mice. CONCLUSIONS: Apart from its insulin sensitizing effect, ACE may have hypoglycemic effects via mechanisms of insulin releasing and alpha-glucosidase inhibition, and thus improves postprandial hyperglycemia and cardiovascular complications. |
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Authors:
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Mei-mei Si; Jian-shu Lou; Chang-Xin Zhou; Juan-Na Shen; Hong-Hai Wu; Bo Yang; Qiao-Jun He; Hao-Shu Wu |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-01-04 |
Journal Detail:
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Title: Journal of ethnopharmacology Volume: 128 ISSN: 1872-7573 ISO Abbreviation: J Ethnopharmacol Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-17 Completed Date: 2010-06-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7903310 Medline TA: J Ethnopharmacol Country: Ireland |
Other Details:
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Languages: eng Pagination: 154-9 Citation Subset: IM |
Copyright Information:
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Copyright (c) 2009 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Institute of Pharmacology & Toxicology and Biochemical Pharmaceutics, College of Pharmaceutical Sciences, Zhejiang University, 388 Yu-hang-tang Road, Hangzhou, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acetates
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chemistry* Acorus / chemistry* Animals Blood Glucose / analysis Cell Line Enzyme Inhibitors / pharmacology* Insulin / secretion* Kinetics Male Mice Mice, Inbred ICR Postprandial Period alpha-Glucosidases / antagonists & inhibitors* |
| Chemical | |
Reg. No./Substance:
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0/Acetates; 0/Blood Glucose; 0/Enzyme Inhibitors; 11061-68-0/Insulin; 141-78-6/ethyl acetate; EC 3.2.1.20/alpha-Glucosidases |
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