| Insulin receptor substrate-1 is expressed at high levels in all cells of the peri-implantation mouse embryo. | |
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MedLine Citation:
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PMID: 9508089 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Insulin and insulinlike growth factors are important for embryonic growth and metabolism. Intracellular transduction for these factors has not been studied in the preimplantation mouse embryo. Peri-implantation mouse embryos synthesize insulinlike growth factor (IGF)-II ligand, insulin receptor, IGF-I receptor, and IGF-II receptor and respond to IGF-II, IGF-I, and insulin metabolically and mitogenically. Maternal tissues in the oviduct and uterus are also sources of IGF-I and insulin. Signaling of IGFs occurs through insulin receptor substrate (IRS)-1 and IRS-2. This paper shows that IRS-1 mRNA and protein are highly expressed in preimplantation mouse embryos, in embryonic cell lines, and in cultured blastocyst outgrowths. IRS-1 mRNA and protein are detected in embryo-derived cell lines cultured to produce the three cell lineages (stem cells, endoderm, and trophoblast cells). IRS-1 mRNA is detected by reverse transcription-polymerase chain reaction (RT-PCR) in the E3.5 blastocyst before implantation and in F9 teratocarcinoma stem cells and parietal endoderm cells. IRS-1 mRNA is detected by Northern blot hybridization at high levels in stem cells and in differentiated progeny of F9 cells and C3H/NE trophectoderm cells. IRS-1 protein was detected in these cell lines and in an overexpressing CHO-IRS-1 fibroblast cell line by immunocytochemistry. Cultured blastocyst outgrowths are a model for implantation events of the trophoblast/placenta lineage and endoderm/yolk sac lineage. In the blastocyst outgrowth, IRS-1 protein is detected in inner cell mass cells (ICM cells), primitive endoderm, parietal endoderm, and trophectoderm cells. These data suggest that IRS-1 is expressed in all cell lineages of the peri-implantation mouse embryo and mediates some effects of insulin and IGFs at this stage. |
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Authors:
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E E Puscheck; E Pergament; Y Patel; J Dreschler; D A Rappolee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Molecular reproduction and development Volume: 49 ISSN: 1040-452X ISO Abbreviation: Mol. Reprod. Dev. Publication Date: 1998 Apr |
Date Detail:
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Created Date: 1998-05-15 Completed Date: 1998-05-15 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 8903333 Medline TA: Mol Reprod Dev Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 386-93 Citation Subset: IM |
Affiliation:
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Department of Obstetrics and Gynecology, Northwestern University Medical School, Chicago, Illinois 60611, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blastocyst / cytology, metabolism* Blotting, Northern Cell Division Cell Line Female Insulin Receptor Substrate Proteins Mice Mice, Inbred C3H Mice, Inbred C57BL Phosphoproteins / biosynthesis* Polymerase Chain Reaction Receptor, Insulin / biosynthesis* Teratocarcinoma / metabolism Tumor Cells, Cultured |
| Grant Support | |
ID/Acronym/Agency:
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HD30739-02/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Insulin Receptor Substrate Proteins; 0/Irs1 protein, mouse; 0/Phosphoproteins; EC 2.7.10.1/Receptor, Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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