Document Detail

Insulin receptor substrate-1 is expressed at high levels in all cells of the peri-implantation mouse embryo.
MedLine Citation:
PMID:  9508089     Owner:  NLM     Status:  MEDLINE    
Insulin and insulinlike growth factors are important for embryonic growth and metabolism. Intracellular transduction for these factors has not been studied in the preimplantation mouse embryo. Peri-implantation mouse embryos synthesize insulinlike growth factor (IGF)-II ligand, insulin receptor, IGF-I receptor, and IGF-II receptor and respond to IGF-II, IGF-I, and insulin metabolically and mitogenically. Maternal tissues in the oviduct and uterus are also sources of IGF-I and insulin. Signaling of IGFs occurs through insulin receptor substrate (IRS)-1 and IRS-2. This paper shows that IRS-1 mRNA and protein are highly expressed in preimplantation mouse embryos, in embryonic cell lines, and in cultured blastocyst outgrowths. IRS-1 mRNA and protein are detected in embryo-derived cell lines cultured to produce the three cell lineages (stem cells, endoderm, and trophoblast cells). IRS-1 mRNA is detected by reverse transcription-polymerase chain reaction (RT-PCR) in the E3.5 blastocyst before implantation and in F9 teratocarcinoma stem cells and parietal endoderm cells. IRS-1 mRNA is detected by Northern blot hybridization at high levels in stem cells and in differentiated progeny of F9 cells and C3H/NE trophectoderm cells. IRS-1 protein was detected in these cell lines and in an overexpressing CHO-IRS-1 fibroblast cell line by immunocytochemistry. Cultured blastocyst outgrowths are a model for implantation events of the trophoblast/placenta lineage and endoderm/yolk sac lineage. In the blastocyst outgrowth, IRS-1 protein is detected in inner cell mass cells (ICM cells), primitive endoderm, parietal endoderm, and trophectoderm cells. These data suggest that IRS-1 is expressed in all cell lineages of the peri-implantation mouse embryo and mediates some effects of insulin and IGFs at this stage.
E E Puscheck; E Pergament; Y Patel; J Dreschler; D A Rappolee
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular reproduction and development     Volume:  49     ISSN:  1040-452X     ISO Abbreviation:  Mol. Reprod. Dev.     Publication Date:  1998 Apr 
Date Detail:
Created Date:  1998-05-15     Completed Date:  1998-05-15     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8903333     Medline TA:  Mol Reprod Dev     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  386-93     Citation Subset:  IM    
Department of Obstetrics and Gynecology, Northwestern University Medical School, Chicago, Illinois 60611, USA.
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MeSH Terms
Blastocyst / cytology,  metabolism*
Blotting, Northern
Cell Division
Cell Line
Insulin Receptor Substrate Proteins
Mice, Inbred C3H
Mice, Inbred C57BL
Phosphoproteins / biosynthesis*
Polymerase Chain Reaction
Receptor, Insulin / biosynthesis*
Teratocarcinoma / metabolism
Tumor Cells, Cultured
Grant Support
Reg. No./Substance:
0/Insulin Receptor Substrate Proteins; 0/Irs1 protein, mouse; 0/Phosphoproteins; EC, Insulin

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