Document Detail

Insulin promotes T cell recovery in a murine model of autoimmune myocarditis.
MedLine Citation:
PMID:  23199322     Owner:  NLM     Status:  MEDLINE    
Glucose-insulin-potassium (GIK) is a useful adjunct to myocarditis. Besides its essential action in energy metabolism, insulin also exerts an anti-inflammatory effect. This study investigated the effect of insulin on myocardial inflammation in experimental autoimmune myocarditis (EAM) in mice and its potential role in T cell regulation. Mice were divided randomly into a normal control group, a saline-treated EAM group and an insulin-treated EAM group. The histopathological changes of myocardium, α-myosin heavy chain (MyHCα)(614-629) antigen-specific autoantibody titre, the serum level of cardiac troponin I (cTnI), mitogen-activated protein kinase (MAPK) family members' activity and content were measured. Furthermore, the phenotype of T lymphocyte subsets in splenocytes was analysed to evaluate the immune status of mice. Insulin reduced serum cTnI of EAM mice on days 14 and 21 (P < 0·05) after immunization, with no changes in blood glucose and autoantibody production. Western blot revealed that extracellular signal-regulated protein kinase (ERK1/2) may be a determining factor in this process. Total ERK1/2 and phospho-ERK1/2 (p-ERK1/2) were both up-regulated in insulin-treated mice after immunization. We also found that insulin treatment promoted T cell recovery without changing the naive-to-memory T-cell ratio; in particular, CD3(+) T cells in insulin-treated mice proliferated more vigorously than in control mice (P < 0·05). We report here for the first time that insulin alleviates myocarditis in the EAM model. These data show that insulin has a direct effect on T cell proliferation in EAM. It is possible that GIK or insulin may assist T cell recovery towards normal in myocarditis, especially for diabetic or hyperglycaemic patients.
Y Zhang; R Zhuang; C Geng; X Cai; W Lei; N Tian; F Gao
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental immunology     Volume:  171     ISSN:  1365-2249     ISO Abbreviation:  Clin. Exp. Immunol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-03     Completed Date:  2013-02-19     Revised Date:  2014-01-10    
Medline Journal Info:
Nlm Unique ID:  0057202     Medline TA:  Clin Exp Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  46-53     Citation Subset:  IM    
Copyright Information:
© 2012 The Authors Clinical and Experimental Immunology © 2012 British Society for Immunology.
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MeSH Terms
Antigens, CD3 / immunology
Autoantibodies / blood
Autoimmune Diseases / drug therapy*,  immunology
Blood Glucose / drug effects
Disease Models, Animal
Insulin / therapeutic use*
Lymphocyte Activation / drug effects,  immunology
Mice, Inbred BALB C
Mitogen-Activated Protein Kinases / analysis
Myocarditis / drug therapy*,  immunology,  pathology
Spleen / enzymology,  immunology
T-Lymphocytes / immunology*
Troponin I / blood
Up-Regulation / drug effects
Ventricular Myosins / immunology
Reg. No./Substance:
0/Antigens, CD3; 0/Autoantibodies; 0/Blood Glucose; 0/Insulin; 0/Troponin I; EC Protein Kinases; EC 3.6.1.-/Ventricular Myosins

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