| Insulin promotes T cell recovery in a murine model of autoimmune myocarditis. | |
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MedLine Citation:
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PMID: 23199322 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glucose-insulin-potassium (GIK) is a useful adjunct to myocarditis. Besides its essential action in energy metabolism, insulin also exerts an anti-inflammatory effect. This study investigated the effect of insulin on myocardial inflammation in experimental autoimmune myocarditis (EAM) in mice and its potential role in T cell regulation. Mice were divided randomly into a normal control group, a saline-treated EAM group and an insulin-treated EAM group. The histopathological changes of myocardium, α-myosin heavy chain (MyHCα)(614-629) antigen-specific autoantibody titre, the serum level of cardiac troponin I (cTnI), mitogen-activated protein kinase (MAPK) family members' activity and content were measured. Furthermore, the phenotype of T lymphocyte subsets in splenocytes was analysed to evaluate the immune status of mice. Insulin reduced serum cTnI of EAM mice on days 14 and 21 (P < 0·05) after immunization, with no changes in blood glucose and autoantibody production. Western blot revealed that extracellular signal-regulated protein kinase (ERK1/2) may be a determining factor in this process. Total ERK1/2 and phospho-ERK1/2 (p-ERK1/2) were both up-regulated in insulin-treated mice after immunization. We also found that insulin treatment promoted T cell recovery without changing the naive-to-memory T-cell ratio; in particular, CD3(+) T cells in insulin-treated mice proliferated more vigorously than in control mice (P < 0·05). We report here for the first time that insulin alleviates myocarditis in the EAM model. These data show that insulin has a direct effect on T cell proliferation in EAM. It is possible that GIK or insulin may assist T cell recovery towards normal in myocarditis, especially for diabetic or hyperglycaemic patients. |
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Authors:
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Y Zhang; R Zhuang; C Geng; X Cai; W Lei; N Tian; F Gao |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 171 ISSN: 1365-2249 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2012-12-03 Completed Date: 2013-02-19 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: England |
Other Details:
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Languages: eng Pagination: 46-53 Citation Subset: IM |
Copyright Information:
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© 2012 The Authors Clinical and Experimental Immunology © 2012 British Society for Immunology. |
Affiliation:
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Department of Physiology, Fourth Military Medical University, Xi'an, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD3 / immunology Autoantibodies / blood Autoimmune Diseases / drug therapy*, immunology Blood Glucose / drug effects Disease Models, Animal Insulin / therapeutic use* Lymphocyte Activation / drug effects, immunology Male Mice Mice, Inbred BALB C Mitogen-Activated Protein Kinases / analysis Myocarditis / drug therapy*, immunology, pathology Spleen / enzymology, immunology T-Lymphocytes / immunology* Troponin I / blood Up-Regulation / drug effects Ventricular Myosins / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD3; 0/Autoantibodies; 0/Blood Glucose; 0/Insulin; 0/Troponin I; EC 2.7.11.24/Mitogen-Activated Protein Kinases; EC 3.6.1.-/Ventricular Myosins |
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