| Insulin metabolism in human adipocytes from subcutaneous and visceral depots. | |
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MedLine Citation:
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PMID: 21036154 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Subjects with the metabolic syndrome (insulin resistance, glucose intolerance, dyslipidemia, hypertension, etc.) have a relative increase in abdominal fat tissue compared to normal individuals and obesity has also been shown to be associated with a decrease in insulin clearance. The majority of the clearance of insulin is due to the action of insulin-degrading enzyme (IDE) and IDE is present throughout all tissues. Since abdominal fat is increased in obesity we hypothesized that IDE may be altered in the different fat depots. Adipocytes were isolated from fat samples obtained from subjects during elective abdominal surgery. Fat samples were taken from subcutaneous (SQ) and visceral (VIS) sites. Insulin metabolism was compared in adipocytes isolated from SQ and VIS fat tissue. Adipocytes from the VIS site degraded more insulin that those from SQ fat tissue. Inhibitors of cathepsins B and D has no effect on the degradation of insulin, while bacitracin, an inhibitor of IDE, inhibited degradation by approx. 33% in both SQ and VIS adipocytes. These data show that insulin metabolism is relatively greater in VIS than in SQ fat tissue and potentially due to IDE. |
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Authors:
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Janet Fawcett; Hairong Sang; Paska A Permana; Jennifer L Levy; William C Duckworth |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-10-29 |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 402 ISSN: 1090-2104 ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 2010 Nov |
Date Detail:
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Created Date: 2010-11-29 Completed Date: 2011-01-13 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: United States |
Other Details:
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Languages: eng Pagination: 762-6 Citation Subset: IM |
Copyright Information:
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Published by Elsevier Inc. |
Affiliation:
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Research Service, Phoenix VA Health Care System, Phoenix, AZ 85012, USA. janet.fawcett@va.gov |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Abdominal Fat
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cytology,
metabolism* Adipocytes / metabolism* Adipose Tissue / cytology, metabolism* Adult Aged Aged, 80 and over Cathepsin B / antagonists & inhibitors, metabolism Cathepsin D / antagonists & inhibitors, metabolism Female Humans Insulin / metabolism* Insulysin / antagonists & inhibitors, metabolism Male Metabolic Syndrome X / metabolism Middle Aged Subcutaneous Tissue / metabolism* |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin; EC 3.4.22.1/CTSB protein, human; EC 3.4.22.1/Cathepsin B; EC 3.4.23.5/CTSD protein, human; EC 3.4.23.5/Cathepsin D; EC 3.4.24.56/Insulysin |
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