| Insulin-like growth factor I stimulates inositol phosphate accumulation, a rise in cytoplasmic free calcium, and proliferation in cultured porcine thyroid cells. | |
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MedLine Citation:
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PMID: 2808386 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Insulin-like growth factor (IGF-I) stimulates thyroid cell proliferation. Using primary cultured porcine thyroid cells, we studied the intracellular pathways that mediate the action of IGF-I on thyroid cell proliferation. IGF-I stimulates inositol phosphate accumulation, a rise in cytoplasmic free calcium [( Ca2+]i), and cell proliferation. Exposure to IGF-I results in a time- and dose-dependent accumulation of inositol monophosphate, inositol bisphosphate, and inositol trisphosphate. IGF-I also increases [Ca2+]i, measured using fura-2, a fluorescent Ca2+ indicator; the IGF-I-induced [Ca2+]i response occurs immediately, reaches a maximum within 1 min, and then slowly declines. IGF-I stimulates thyroid cell proliferation, stimulates thymidine incorporation, and increases cell numbers. The IGF-I-induced inositol phosphate accumulation and [Ca2+]i response parallel thyroid cell proliferation in a dose-dependent manner; the maximal response is observed at a concentration of 100 ng/ml IGF-I, with half-maximal stimulation at approximately 10 ng/ml. Inositol phosphate accumulation and [Ca2+]i response after IGF-I stimulation may function as intracellular messengers for thyroid cell proliferation. This report may constitute the first demonstration of IGF-I-stimulated inositol phosphate accumulation and [Ca2+]i response in the cells. |
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Authors:
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N Takasu; M Takasu; I Komiya; Y Nagasawa; T Asawa; Y Shimizu; T Yamada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 264 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1989 Nov |
Date Detail:
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Created Date: 1989-11-28 Completed Date: 1989-11-28 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 18485-8 Citation Subset: IM |
Affiliation:
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Department of Gerontology, Endocrinology and Metabolism, School of Medicine, Shinshu University, Nagano-ken, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Benzofurans Calcium / metabolism* Cell Division / drug effects Cells, Cultured Cytoplasm / metabolism Dose-Response Relationship, Drug Fluorescent Dyes Fura-2 Inositol Phosphates / metabolism* Insulin-Like Growth Factor I / administration & dosage, pharmacology* Kinetics Somatomedins / pharmacology* Spectrometry, Fluorescence Swine Thyroid Gland / cytology, drug effects, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Benzofurans; 0/Fluorescent Dyes; 0/Inositol Phosphates; 0/Somatomedins; 67763-96-6/Insulin-Like Growth Factor I; 7440-70-2/Calcium; 96314-98-6/Fura-2 |
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