| Insulin-like growth factor I in the pancreas of normal and diabetic adult rats. | |
| | |
MedLine Citation:
|
PMID: 2465668 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Insulin-like growth factor I (IGF-I, somatomedin C) was mapped by immunocytochemistry in the pancreas of normal and experimentally influenced rats. The polyclonal IGF-I antiserum K 37 was characterized and demonstrated to be specific. In the exocrine pancreas some duct cells showed IGF-I immunoreactivity, other components being negative. The three main endocrine cell types in the islets of Langerhans were IGF-I immunoreactive, most strikingly the D cells. Hypophysectomy resulted in loss of IGF-I immunoreactivity in all three endocrine cell types, i.e. D, A and B cells, while the levels of somatostatin, glucagon and insulin, respectively, remained unchanged. Starvation seemed to increase and feeding to decrease the IGF-I immunoreactivity in the B cells. Cysteamine pre-treatment reduced the normally intense IGF-I and somatostatin immunoreactivities in the D cells. In rats made diabetic with alloxan or streptozotocin, the B cells were irreversibly damaged and lost both their insulin and IGF-I immunoreactivities, while the IGF-I immunoreactivity was increased in A cells; the D cells remained unchanged. The concentrations of IGF-I mRNA in the pancreas were almost equal in normal and alloxan diabetic rats as were the concentrations of extractable IGF-I. We conclude that IGF-I immunoreactive material can be demonstrated in adult animals in all endocrine islet cells, most prominently in the D cells. The expression of IGF-I immunoreactivity is in part under pituitary control. In the adult rat only one islet cell type synthesizes IGF-I immunoreactive material, i.e. the D cells, while, in contrast, the B cells are likely to be a major IGF-I source in fetal and neonatal islets. |
| | |
Authors:
|
H A Hansson; D Edwall; B Löwenadler; G Norstedt; S Paleus; A Skottner |
Related Documents
:
|
7687588 - Characterization of mouse urothelial cell lines in different phases of transitional-cel... 20599498 - Rgs19 enhances cell proliferation through its c-terminal pdz motif. 15172968 - Multiple myeloma cells catalyze hepatocyte growth factor (hgf) activation by secreting ... 20876298 - Ime1 and ime2 are required for pseudohyphal growth of saccharomyces cerevisiae on nonfe... 22012578 - Andrographolide induces cell cycle arrest and apoptosis in human rheumatoid arthritis f... 7685298 - Regional differences between various axial segments of the avian neural crest regarding... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Acta physiologica Scandinavica Volume: 132 ISSN: 0001-6772 ISO Abbreviation: Acta Physiol. Scand. Publication Date: 1988 Apr |
Date Detail:
|
Created Date: 1989-03-27 Completed Date: 1989-03-27 Revised Date: 2003-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 0370362 Medline TA: Acta Physiol Scand Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 569-76 Citation Subset: IM |
Affiliation:
|
Department of Histology, University of Gothenburg, Sweden. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Diabetes Mellitus, Experimental / metabolism* Hypophysectomy Immunohistochemistry Insulin-Like Growth Factor I / metabolism* Islets of Langerhans / metabolism* Nucleic Acid Hybridization Pancreas / metabolism* RNA Radioimmunoassay Rats Rats, Inbred Strains Somatomedins / metabolism* |
| Chemical | |
Reg. No./Substance:
|
0/Somatomedins; 63231-63-0/RNA; 67763-96-6/Insulin-Like Growth Factor I |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Increased behavioural response to intrathecal substance P after intracerebroventricular 5,7-dihydrox...
Next Document: Effects of nimodipine, Bay K 8644 and pinacidil on alpha 1- and alpha 2-adrenoceptor-mediated vasoco...