Document Detail

Insulin-like growth factor binding protein-1 expression in baboon endometrial stromal cells: regulation by filamentous actin and requirement for de novo protein synthesis.
MedLine Citation:
PMID:  9927334     Owner:  NLM     Status:  MEDLINE    
Stromal fibroblasts in the primate endometrium undergo dramatic morphological and biochemical changes in response to pregnancy. This transformation is characterized by the expression of insulin-like growth factor binding protein-1 (IGFBP-1). Stromal cells from the baboon endometrium of nonpregnant animals were cultured and subsequently treated with cytochalasin D to disrupt actin filaments. In response to cytochalasin D treatment, cells contracted and became rounded as early as 10 min after the initiation of treatment. When cytochalasin D was removed, cells reverted back to their original fibroblastic shape within 1 h. After cells were treated with cytochalasin D for 5 h, addition of (Bu)2cAMP and/or hormones (estradiol, medroxyprogesterone acetate, and relaxin) resulted in the expression of IGFBP-1 messenger RNA and protein within 24 h. Cells with an intact cytoskeleton did not express detectable levels of IGFBP-1 in response to hormones and/or (Bu)2cAMP. Furthermore, the addition of cycloheximide inhibited expression of IGFBP-1 in cytochalasin D-treated cells. Stromal cells were also isolated from early pregnant and simulated pregnant animals. Within 48 h, cells from both the pregnant and simulated pregnant animals produced IGFBP-1 in response to hormones and/or (Bu)2cAMP. In these studies, IGFBP-1 expression was also inhibited by cycloheximide. These studies suggest that induction of IGFBP-1 requires an intermediary protein and that alterations in the cytoskeleton may be involved.
J J Kim; R C Jaffe; A T Fazleabas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Endocrinology     Volume:  140     ISSN:  0013-7227     ISO Abbreviation:  Endocrinology     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-02-23     Completed Date:  1999-02-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375040     Medline TA:  Endocrinology     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  997-1004     Citation Subset:  AIM; IM    
Department of Obstetrics and Gynecology, University of Illinois at Chicago, 60212-7313, USA.
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MeSH Terms
Actins / physiology*
Cycloheximide / pharmacology
Cytochalasin D / pharmacology
Endometrium / cytology,  metabolism*
Insulin-Like Growth Factor Binding Protein 1 / metabolism*
Microtubules / metabolism
Nucleic Acid Synthesis Inhibitors / pharmacology
Protein Biosynthesis
Protein Synthesis Inhibitors / pharmacology
Stromal Cells / cytology,  metabolism*
Reg. No./Substance:
0/Actins; 0/Insulin-Like Growth Factor Binding Protein 1; 0/Nucleic Acid Synthesis Inhibitors; 0/Protein Synthesis Inhibitors; 22144-77-0/Cytochalasin D; 66-81-9/Cycloheximide

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