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Insulin-like growth factor II activates mitogenic signaling in pancreatic cancer cells via IRS-1.
MedLine Citation:
PMID:  21541539     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Insulin-like growth factor-II (IGF-II) action and expression were examined in 3 human pancreatic cancer cell lines. IGF-II expression was also studied in 17 normal and 12 malignant pancreatic tissues. IGF-II enhanced the growth of all 3 cell lines. In COLO-357 and PANC-1 cells, one-half maximal stimulation occurred at 0.3 and 0.4 nM IGF-II, respectively. In ASPC-1 cells, one-half maximal stimulation occurred at 0.9 nM IGF-II. A monoclonal antibody (alpha IR3) that blocks ligand binding to the insulin-like growth factor I (IGF-I) receptor (IGF-IR) inhibited IGF-II-mediated growth stimulation, and IGF-II enhanced insulin-receptor substrate 1 (IRS-1) phosphorylation. IGF-II mRNA transcripts were present in COLO-357 cells, in 4 of 17 normal human pancreatic tissues, and in 8 of the 12 cancer samples. By immunohistochemistry, IGF-II was present in the islets of both normal and malignant pancreatic tissues, and occasionally in the cancer cells within the tumor mass. These findings indicate that IGF-II acts via IGF-IR to enhance mitogenic signaling in pancreatic cancer cells and suggest that islet-derived IGF-II may contribute to pancreatic cancer cell growth in vivo.
Authors:
U Bergmann; H Funatomi; M Kornmann; T Ishiwata; H Beger; M Korc
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of oncology     Volume:  9     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  2011-05-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  487-92     Citation Subset:  -    
Affiliation:
UNIV CALIF IRVINE,DIV ENDOCRINOL DIABET & METAB,IRVINE,CA 92717. UNIV ULM,DEPT GEN SURG,D-89075 ULM,GERMANY.
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