Document Detail

Insulin-like growth factor II activates mitogenic signaling in pancreatic cancer cells via IRS-1.
MedLine Citation:
PMID:  21541539     Owner:  NLM     Status:  In-Data-Review    
Insulin-like growth factor-II (IGF-II) action and expression were examined in 3 human pancreatic cancer cell lines. IGF-II expression was also studied in 17 normal and 12 malignant pancreatic tissues. IGF-II enhanced the growth of all 3 cell lines. In COLO-357 and PANC-1 cells, one-half maximal stimulation occurred at 0.3 and 0.4 nM IGF-II, respectively. In ASPC-1 cells, one-half maximal stimulation occurred at 0.9 nM IGF-II. A monoclonal antibody (alpha IR3) that blocks ligand binding to the insulin-like growth factor I (IGF-I) receptor (IGF-IR) inhibited IGF-II-mediated growth stimulation, and IGF-II enhanced insulin-receptor substrate 1 (IRS-1) phosphorylation. IGF-II mRNA transcripts were present in COLO-357 cells, in 4 of 17 normal human pancreatic tissues, and in 8 of the 12 cancer samples. By immunohistochemistry, IGF-II was present in the islets of both normal and malignant pancreatic tissues, and occasionally in the cancer cells within the tumor mass. These findings indicate that IGF-II acts via IGF-IR to enhance mitogenic signaling in pancreatic cancer cells and suggest that islet-derived IGF-II may contribute to pancreatic cancer cell growth in vivo.
U Bergmann; H Funatomi; M Kornmann; T Ishiwata; H Beger; M Korc
Related Documents :
2503989 - Studies on the mechanisms of the synergistic effects of ethambutol and other antibacter...
2299239 - Removal of excess nuclear protein from cells heated in different physiological states.
16657879 - Microcalorimetric measurements of the heat evolution and its sensitivity to dinitrophen...
9251209 - Effects of several factors on the heat-shock-induced thermotolerance of listeria monocy...
1375619 - Induction of 72-kd heat shock protein and cytoskeleton damage by cytotoxic prostaglandi...
8615589 - Experimental otitis media with moraxella (branhamella) catarrhalis.
12713429 - Purification of mouse primordial germ cells by nycodenz.
22125689 - A review of clinical profile in sickle cell traits.
24835429 - Biosynthesis of gold nanoparticles and related cytotoxicity evaluation using a549 cells.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  International journal of oncology     Volume:  9     ISSN:  1019-6439     ISO Abbreviation:  Int. J. Oncol.     Publication Date:  1996 Sep 
Date Detail:
Created Date:  2011-05-04     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9306042     Medline TA:  Int J Oncol     Country:  Greece    
Other Details:
Languages:  eng     Pagination:  487-92     Citation Subset:  -    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Chromosomal assignment of three human melanocyte-specific genes.
Next Document:  Cell-cycle regulator cyclin D1 mRNA and protein overexpression occurs in primary malignant gliomas.