| Insulin-like growth factor I (IGF I) stimulates DNA synthesis in fetal rat brain cell cultures. | |
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MedLine Citation:
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PMID: 6133596 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Addition of insulin, IGF I or IGF II to serum-free cultures of fetal rat brain cells (gestation day 15/16) significantly stimulates DNA synthesis. The dose-response curves show that IGF I is more potent than insulin; half maximal stimulation of [3H]thymidine incorporation is obtained at about 0.4 nM IGF I and 14 nM insulin, respectively. Cultures initiated 2 days later (gestation day 17/18) showed a decreased responsiveness to both peptides. No additive effect was observed after combined addition of both peptides at near-maximal doses. Both peptides show a latency of action of about 12-18 h. In the presence of either IGF or insulin, neuronal as well as glial enzymes are increased, suggesting that neuronal and glial precursor cell division is influenced. IGF I and IGF II interact with a specific binding site for which insulin competes very weakly; however IGF I and IGF II bind with relatively high affinity to the insulin specific binding site. The present results support the hypothesis that both insulin and IGF stimulate mitotic activity by interacting with specific somatomedin receptors and suggest a physiological role of IGF in the developing brain. |
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Authors:
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D Lenoir; P Honegger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Brain research Volume: 283 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1983 Apr |
Date Detail:
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Created Date: 1983-07-08 Completed Date: 1983-07-08 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 205-13 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / cytology, drug effects* Cell Differentiation / drug effects* Culture Techniques DNA / biosynthesis* Dose-Response Relationship, Drug Female Fetus Insulin / metabolism, pharmacology* Neurons / cytology Neurotransmitter Agents / metabolism Peptides / metabolism, pharmacology* Pregnancy Rats Rats, Inbred Strains Receptors, Cell Surface / metabolism Receptors, Somatomedin Somatomedins / metabolism, pharmacology* Thymidine / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Neurotransmitter Agents; 0/Peptides; 0/Receptors, Cell Surface; 0/Receptors, Somatomedin; 0/Somatomedins; 11061-68-0/Insulin; 50-89-5/Thymidine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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