Document Detail


Insulin-like growth factor-1 (IGF-1) specifically binds to bovine lens epithelial cells and increases the number of fibronectin receptor sites.
MedLine Citation:
PMID:  7924417     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The purpose of the study was to determine the quantitative characteristics of IGF-1 binding sites in lens cells and to investigate its ability to modulate cell growth, in particular by inducing integrin expression. Studies were carried out in bovine lens epithelial cells in culture. IGF-1 receptor binding parameters were measured from saturation experiments with 125I-IGF-1. Scatchard plot indicates one class of high affinity sites (Kd = 2.5 +/- 1.5 nM). In addition, we showed that this growth factor was synthesized and released by lens cells. The characteristics of the receptor sites are in accordance with the effects of the growth factor (e.g. stimulation of DNA synthesis) in the range of nM concentrations. Moreover, by incubating cells with IGF-1 (12 nM) for 40 hr we demonstrated that the expression of integrin, the fibronectin receptor, was activated (N = 885 +/- 169 x 10(3) sites/cell vs N = 453 +/- 105 x 10(3) sites/cell in control cultures) without modification of its affinity (Kd congruent to 16 x 10(-8) M). These new data emphasize the role of IGF-1 in the regulation of migration, proliferation and differentiation of mature lens cells.
Authors:
F Palmade; O Sechoy-Chambon; C Coquelet; C Bonne
Related Documents :
15123067 - Microbial-gut interactions in health and disease. epithelial cell responses.
7756447 - Cell origin and paracrine control of interstitial collagenase in the guinea pig uterine...
11592337 - Effects of antibiotics on morphologic characteristics and migration of canine corneal e...
19487817 - Emt: when epithelial cells decide to become mesenchymal-like cells.
18715777 - Negative dielectrophoretic patterning with different cell types.
19490637 - Chemosensitivity of radioresistant cells in the multicellular spheroids of a549 lung ad...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current eye research     Volume:  13     ISSN:  0271-3683     ISO Abbreviation:  Curr. Eye Res.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-11-17     Completed Date:  1994-11-17     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8104312     Medline TA:  Curr Eye Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  531-7     Citation Subset:  IM    
Affiliation:
Laboratoire de Physiologie Cellulaire, Université Montpellier I, France.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Cattle
Cell Differentiation
Cell Division
Cell Movement
Cells, Cultured
DNA / biosynthesis
Epithelium / metabolism
Fibronectins / metabolism
Insulin-Like Growth Factor I / metabolism*,  pharmacology
Lens, Crystalline / drug effects,  metabolism*
Receptor, IGF Type 1 / metabolism
Receptors, Fibronectin / metabolism*
Thymidine / metabolism
Chemical
Reg. No./Substance:
0/Fibronectins; 0/Receptors, Fibronectin; 50-89-5/Thymidine; 67763-96-6/Insulin-Like Growth Factor I; 9007-49-2/DNA; EC 2.7.10.1/Receptor, IGF Type 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Molecular identity and calmodulin-mediated regulation of the taurine transporter in a human retinal ...
Next Document:  The existence of protein kinase C in cone photoreceptors in the rat retina.