| Insulin increases H2O2-induced pancreatic beta cell death. | |
| | |
MedLine Citation:
|
PMID: 20544287 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Insulin resistance results, in part, from impaired insulin signaling in insulin target tissues. Consequently, increased levels of insulin are necessary to control plasma glucose levels. The effects of elevated insulin levels on pancreatic beta (β) cell function, however, are unclear. In this study, we investigated the possibility that insulin may influence survival of pancreatic β cells. Studies were conducted on RINm, RINm5F and Min-6 pancreatic β-cells. Cell death was induced by treatment with H(2)O(2), and was estimated by measurements of LDH levels, viability assay (Cell-Titer Blue), propidium iodide staining and FACS analysis, and mitochondrial membrane potential (JC-1). In addition, levels of cleaved caspase-3 and caspase activity were determined. Treatment with H(2)O(2) increased cell death; this effect was increased by simultaneous treatment of cells with insulin. Insulin treatment alone caused a slight increase in cell death. Inhibition of caspase-3 reduced the effect of insulin to increase H(2)O(2)-induced cell death. Insulin increased ROS production by pancreatic β cells and increased the effect of H(2)O(2). These effects were increased by inhibition of IR signaling, indicative of an effect independent of the IR cascade. We conclude that elevated levels of insulin may act to exacerbate cell death induced by H(2)O(2) and, perhaps, other inducers of apoptosis. |
| | |
Authors:
|
S R Sampson; E Bucris; M Horovitz-Fried; A Parnas; S Kahana; G Abitbol; M Chetboun; T Rosenzweig; C Brodie; S Frankel |
Related Documents
:
|
15372107 - Pdx-1 haploinsufficiency limits the compensatory islet hyperplasia that occurs in respo... 8231837 - Selective impairment of the cytoplasmic ca2+ response to glucose in pancreatic beta cel... 17073627 - Current progress in non-invasive imaging of beta cell mass of the endocrine pancreas. 12219087 - Regulation of insulin action and pancreatic beta-cell function by mutated alleles of th... 18249457 - Early manifestation of macrovasculopathy in newly diagnosed never treated type ii diabe... 16111757 - Vascular response of the bulbar conjunctiva to diabetes and elevated blood pressure. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Apoptosis : an international journal on programmed cell death Volume: 15 ISSN: 1573-675X ISO Abbreviation: Apoptosis Publication Date: 2010 Oct |
Date Detail:
|
Created Date: 2010-10-01 Completed Date: 2010-12-30 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9712129 Medline TA: Apoptosis Country: United States |
Other Details:
|
Languages: eng Pagination: 1165-76 Citation Subset: IM |
Affiliation:
|
Mina and Everard Goodman Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan, Israel. ssampson@013.net |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Apoptosis* Blotting, Western Caspases / metabolism Cell Survival / drug effects Cells, Cultured Flow Cytometry Hydrogen Peroxide / toxicity* Insulin / metabolism* Insulin Resistance Insulin-Secreting Cells / drug effects*, metabolism* Mice Oxidative Stress Rats Reactive Oxygen Species / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Reactive Oxygen Species; 11061-68-0/Insulin; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Teriflunomide encourages cytostatic and apoptotic effects in premalignant and malignant cutaneous ke...
Next Document: Expanded newborn screening: social and ethical issues.