Document Detail

Insulin facilitation of muscle protein synthesis following resistance exercise in hindlimb-suspended rats is independent of a rapamycin-sensitive pathway.
MedLine Citation:
PMID:  15304378     Owner:  NLM     Status:  MEDLINE    
Hindlimb suspension (HS) results in rapid losses of muscle mass, which may in part be explained by attenuated rates of protein synthesis. Mammalian target of rapamycin (mTOR) regulates protein synthesis and has been implicated as a potential mediator of the muscle mass decrement with HS. This study examined the effect of resistance exercise, a muscle hypertrophy stimulant, on rates of protein synthesis after 4 days of HS in mature male Sprague-Dawley rats. Flywheel resistance exercise (2 sets x 25 repetitions) was conducted on days 2 and 4 of HS (HSRE). Sixteen hours after the last exercise bout, soleus muscles were assessed for in vitro rates of protein synthesis, with and without insulin (signaling agonist) and/or rapamycin (mTOR inhibitor). Results demonstrated that soleus mass was reduced (P < 0.05) with HS, but this loss of mass was not observed (P > 0.05) with HSRE. Muscle protein synthesis was diminished (P < 0.05) with HS, with or without insulin. HSRE also had reduced rates of synthesis without insulin; however, insulin administration yielded higher (P < 0.05) rates in HSRE compared with HS or control. Rapamycin diminished protein synthesis in all groups (P < 0.05), but insulin rescued synthesis rates in HS and HSRE to levels similar to insulin alone for each group, suggesting that alternate signaling pathways develop to increase protein synthesis with HS. These results demonstrate that the capacity for an augmented anabolic response to resistance exercise is maintained after 4 days of HS and is independent of a rapamycin-sensitive pathway.
James D Fluckey; Esther E Dupont-Versteegden; Micheal Knox; Dana Gaddy; Per A Tesch; Charlotte A Peterson
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.     Date:  2004-08-10
Journal Detail:
Title:  American journal of physiology. Endocrinology and metabolism     Volume:  287     ISSN:  0193-1849     ISO Abbreviation:  Am. J. Physiol. Endocrinol. Metab.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-04     Completed Date:  2004-12-16     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  100901226     Medline TA:  Am J Physiol Endocrinol Metab     Country:  United States    
Other Details:
Languages:  eng     Pagination:  E1070-5     Citation Subset:  IM; S    
Nutrition, Metabolism and Exercise Laboratory, University of Arkansas for Medical Sciences, 4301 W. Markham, Slot 806, Little Rock, AR 72205, USA.
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MeSH Terms
Hindlimb Suspension*
Insulin / pharmacology*
Muscle Proteins / biosynthesis*
Muscle, Skeletal / anatomy & histology,  physiology*
Organ Size
Physical Exertion / physiology*
Protein Kinases / drug effects,  physiology*
Rats, Sprague-Dawley
Sirolimus / pharmacology*
Time Factors
Grant Support
Reg. No./Substance:
0/Muscle Proteins; 11061-68-0/Insulin; 53123-88-9/Sirolimus; EC 2.7.-/Protein Kinases; EC 2.7.1.-/mTOR protein

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