Document Detail

Insulin is essential for in vitro chondrogenesis of mesenchymal progenitor cells and influences chondrogenesis in a dose-dependent manner.
MedLine Citation:
PMID:  23229799     Owner:  NLM     Status:  MEDLINE    
PURPOSE: Insulin is a commonly used additive in chondrogenic media for differentiating mesenchymal stem cells (MSCs). The indispensability of other bioactive factors like TGF-β or dexamethasone in these medium formulations has been shown, but the role of insulin is unclear. The purpose of this study was to investigate whether insulin is essential for MSC chondrogenesis and if there is a dose-dependent effect of insulin on MSC chondrogenesis.
METHODS: We cultivated human MSCs in pellet culture in serum-free chondrogenic medium with insulin concentrations between 0 and 50 μg/ml and assessed the grade of chondrogenic differentiation by histological evaluation and determination of glycosaminoglycan (GAG), total collagen and DNA content. We further tested whether insulin can be delivered in an amount sufficient for MSC chondrogenesis via a drug delivery system in insulin-free medium.
RESULTS: Chondrogenesis was not induced by standard chondrogenic medium without insulin and the expression of cartilage differentiation markers was dose-dependent at insulin concentrations between 0 and 10 μg/ml. An insulin concentration of 50 μg/ml had no additional effect compared with 10 μg/ml. Insulin was delivered by a release system into the cell culture under insulin-free conditions in an amount sufficient to induce chondrogenesis.
CONCLUSIONS: Insulin is essential for MSC chondrogenesis in this system and chondrogenic differentiation is influenced by insulin in a dose-dependent manner. Insulin can be provided in a sufficient amount by a drug delivery system. Therefore, insulin is a suitable and inexpensive indicator substance for testing drug release systems in vitro.
Michael B Mueller; Torsten Blunk; Bernhard Appel; Angelika Maschke; Achim Goepferich; Johannes Zellner; Carsten Englert; Lukas Prantl; Richard Kujat; Michael Nerlich; Peter Angele
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Publication Detail:
Type:  In Vitro; Journal Article     Date:  2012-12-11
Journal Detail:
Title:  International orthopaedics     Volume:  37     ISSN:  1432-5195     ISO Abbreviation:  Int Orthop     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-31     Completed Date:  2013-05-27     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  7705431     Medline TA:  Int Orthop     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  153-8     Citation Subset:  IM    
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MeSH Terms
Analysis of Variance
Cell Differentiation
Cells, Cultured
Chondrogenesis / drug effects*
Collagen / metabolism
DNA / metabolism
Dose-Response Relationship, Drug
Drug Delivery Systems
Enzyme-Linked Immunosorbent Assay
Glycosaminoglycans / metabolism
Insulin / administration & dosage,  pharmacology*
Mesenchymal Stromal Cells / drug effects*
Staining and Labeling
Reg. No./Substance:
0/Glycosaminoglycans; 0/Insulin; 9007-34-5/Collagen; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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