Document Detail

Insulin effect on isolated rat hepatocytes: diacylglycerol-phosphatidic acid interrelationship.
MedLine Citation:
PMID:  1420324     Owner:  NLM     Status:  MEDLINE    
It is widely accepted that insulin action does not involve inositol phospholipid hydrolysis through the stimulation of a phosphatidylinositol-specific phospholipase C (PI-PLC). This consideration prompted us to investigate the insulin effect on the mechanism leading to the accumulation of diacylglycerol (DAG) and phosphatidic acid (PA) in rat hepatocytes. Basically, insulin induces: (i) a significant increase of both [3H]glycerol and fatty acid labelling of DAG; (ii) a significant increase of PA labelling preceding DAG labelling and paralleled by a decrease of phosphatidylcholine (PC) labelling. These observations, which suggest an insulin-dependent involvement of a phospholipase D, are strengthened by the increase of PC-derived phosphatidylethanol in presence of ethanol. Finally, the observation that the PA levels do not return to basal suggests that other mechanisms different from PC hydrolysis, such as the stimulation of direct synthesis of PA, may be activated.
P M Baldini; A Zannetti; V Donchenko; L Dini; P Luly
Related Documents :
509374 - Hepatic glycogen formation by direct uptake of glucose following oral glucose loading i...
9341134 - Metabolic impact of adenovirus-mediated overexpression of the glucose-6-phosphatase cat...
11238784 - In vivo insulin regulation of skeletal muscle glycogen synthase in calorie-restricted a...
12160694 - Glycogenosis type i and diabetes mellitus: a common mechanism for renal dysfunction?
10794644 - Catalase in the heat-induced chilling tolerance of cold-stored hybrid fortune mandarin ...
23549084 - Hepatic glucose sensing is required to preserve β cell glucose competence.
Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1137     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1992 Oct 
Date Detail:
Created Date:  1992-12-08     Completed Date:  1992-12-08     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  208-14     Citation Subset:  IM    
Department of Biology, University of Rome, Tor Vergata, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Cell Membrane / metabolism
Diglycerides / metabolism*
Fatty Acids / metabolism
Insulin / physiology*
Liver / cytology,  metabolism*
Phosphatidic Acids / metabolism*
Phosphatidylcholines / metabolism
Rats, Wistar
Reg. No./Substance:
0/Diglycerides; 0/Fatty Acids; 0/Phosphatidic Acids; 0/Phosphatidylcholines; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Poly(ADP-ribosyl)ation of chromatin in an in-vitro poly(ADP-ribose)-turnover system.
Next Document:  The mitochondrial carnitine carrier: characterization of SH-groups relevant for its transport functi...