Document Detail

Insulin dependant diabetes mellitus: implications for male reproductive function.
MedLine Citation:
PMID:  17478459     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Diabetes mellitus (DM) is increasing in men of reproductive age. Despite this, the prevalence of diabetes in men attending fertility clinics is largely unknown. Furthermore, studies examining the effects of DM on sperm fertility potential have been limited to conventional semen analysis. METHODS: Conventional semen analysis (semen volume, sperm count, motility and morphology) was performed for 27 diabetic (mean age 34+/-2 years) and 29 non-diabetic subjects (control group, men undergoing routine infertility investigations, mean age 33+/-1 years). Nuclear DNA (nDNA) fragmentation was assessed using the alkaline Comet assay and mitochondrial DNA (mtDNA) deletions by Long-PCR. RESULTS: Other than a small, but significant, reduction in semen volume in diabetic men (2.6 versus 3.3 ml; P<0.05), conventional semen parameters did not differ significantly from control subjects. Diabetic subjects had significantly higher mean nDNA fragmentation (53 versus 32%; P<0.0001) and median number of mtDNA deletions (4 versus 3; P<0.05) compared with control subjects. CONCLUSIONS: Diabetes is associated with increased sperm nuclear and mtDNA damage that may impair the reproductive capability of these men.
I M Agbaje; D A Rogers; C M McVicar; N McClure; A B Atkinson; C Mallidis; S E M Lewis
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-05-03
Journal Detail:
Title:  Human reproduction (Oxford, England)     Volume:  22     ISSN:  0268-1161     ISO Abbreviation:  Hum. Reprod.     Publication Date:  2007 Jul 
Date Detail:
Created Date:  2007-06-21     Completed Date:  2007-09-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701199     Medline TA:  Hum Reprod     Country:  England    
Other Details:
Languages:  eng     Pagination:  1871-7     Citation Subset:  IM    
Reproductive Medicine Research Group, Centre for Clinical and Population Sciences, Queen's University of Belfast, Institute of Clinical Science, and Regional Centre for Endocrinology and Diabetes, Royal Victoria Hospital, UK.
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MeSH Terms
Comet Assay
DNA Fragmentation
DNA, Mitochondrial / metabolism
Diabetes Complications*
Diabetes Mellitus, Type 1 / complications,  diagnosis*
Electrophoresis, Agar Gel
Infertility, Male / diagnosis*
Middle Aged
Polymerase Chain Reaction
Sperm Count
Spermatozoa / metabolism
Reg. No./Substance:
0/DNA, Mitochondrial

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