Document Detail

Insulin and cyclic AMP act at different levels on transcription of the L-type pyruvate kinase gene.
MedLine Citation:
PMID:  9395079     Owner:  NLM     Status:  MEDLINE    
We previously demonstrated that, in hepatocytes in primary culture, the role of insulin on induction of L-type pyruvate kinase (L-PK) gene expression was mainly to induce glucokinase synthesis, needed for glucose phosphorylation to glucose 6-phosphate. However, we show here that when hepatocytes have been isolated from rats starved for 72 h, glucose and constitutive glucokinase expression was not sufficient to fully stimulate the L-PK promoter, low insulin concentrations being still required. In addition, activation remains sensitive to cAMP inhibition, but cannot be reproduced in the absence of insulin by a competitive cAMP antagonist. We propose that both insulin and cAMP act on expression of the L-PK gene at, at least, two levels: positive and negative regulation of glucokinase gene expression, and more downstream levels.
M H Cuif; B Doiron; A Kahn
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  FEBS letters     Volume:  417     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  1997 Nov 
Date Detail:
Created Date:  1998-01-07     Completed Date:  1998-01-07     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  81-4     Citation Subset:  IM    
Institut Cochin de Génétique Moléculaire, U. 129 INSERM, Université René Descartes, Paris, France.
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MeSH Terms
Cells, Cultured
Cyclic AMP / metabolism*,  pharmacology
Gene Expression Regulation
Glucokinase / metabolism
Glucose / metabolism
Insulin / metabolism*,  pharmacology
Liver / cytology
Promoter Regions, Genetic*
Pyruvate Kinase / genetics*
Rats, Sprague-Dawley
Transcription, Genetic*
Transcriptional Activation
Reg. No./Substance:
11061-68-0/Insulin; 50-99-7/Glucose; 60-92-4/Cyclic AMP; EC; EC Kinase

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