Document Detail

Insulin attenuates the cytokine response in a burn wound infection model.
MedLine Citation:
PMID:  17224797     Owner:  NLM     Status:  MEDLINE    
A massive burn is one of the most serious injuries resulting in major imbalances of the immune system. The aftermath of a burn is frequently complicated by infections and septic events that additionally increase mortality and morbidity. The aim of the present study was to investigate if insulin attenuates the cytokine response of burned mice challenged with Pseudomonas. Male mice (C57/BL/6) received a full thickness burn of 35% of their total body surface area. Mice received 5 IU/kg insulin i.p. or an equal volume of saline for 5 days after burn. Mice were challenged with 5x10 colony forming units Pseudomonas aeruginosa intraperitoneally. Serum was harvested 6 h after the bacterial challenge, and 18 serum cytokines were measured using the Bio-Plex suspension array system (Bio-Rad, Hercules, Calif). All 18 cytokines were elevated after the Pseudomonas challenge. However, mice treated with insulin showed significantly lower proinflammatory cytokine concentrations of interleukin 5, interleukin 6, and keratinocyte-derived chemokine after the Pseudomonas infection when compared with placebo-treated mice (P<0.05). In contrast, serum concentrations of G-CSF were significantly higher in insulin-treated animals when compared with placebo (P<0.05). We conclude, that insulin treatment selectively modulates specific cytokines in a burn wound infection model.
Rene Przkora; David N Herndon; Celeste C Finnerty; Marc G Jeschke
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Shock (Augusta, Ga.)     Volume:  27     ISSN:  1073-2322     ISO Abbreviation:  Shock     Publication Date:  2007 Feb 
Date Detail:
Created Date:  2007-01-16     Completed Date:  2007-04-05     Revised Date:  2009-11-03    
Medline Journal Info:
Nlm Unique ID:  9421564     Medline TA:  Shock     Country:  United States    
Other Details:
Languages:  eng     Pagination:  205-8     Citation Subset:  IM    
Shriners Hospital for Children and Department of Surgery, University of Texas Medical Branch, Galveston, TX 77550, USA.
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MeSH Terms
Burns / blood*,  microbiology,  pathology
Cytokines / blood*
Disease Models, Animal
Hypoglycemic Agents / pharmacology*
Insulin / pharmacology*
Pseudomonas Infections / blood*,  pathology
Pseudomonas aeruginosa
Wound Infection / blood*,  microbiology,  pathology
Grant Support
5 T32 GM0825-15/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Cytokines; 0/Hypoglycemic Agents; 11061-68-0/Insulin

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