| Insulin alone or with captopril: effects on signaling pathways (AKT and AMPK) and oxidative balance after ischemia-reperfusion in isolated hearts. | |
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MedLine Citation:
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PMID: 22029532 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Insulin and the inhibition of the renin-angiotensin system have independent benefits for ischemia-reperfusion injury, but their combination has not been tested. Our aim was to evaluate the effects of insulin+captopril on insulin/angiotensin signaling pathways and cardiac function in the isolated heart subjected to ischemia-reperfusion. Isolated hearts were perfused (Langendorff technique) with Krebs-Henseleit (KH) buffer for 25 min. Global ischemia was induced (20 min), followed by reperfusion (30 min) with KH (group KH), KH+angiotensin-I (group A), KH+angiotensin-I+captopril (group AC), KH+insulin (group I), KH+insulin+angiotensin-I (group IA), or KH+insulin+angiotensin-I+captopril (group IAC). Group A had a 24% reduction in developed pressure and an increase in end-diastolic pressure vs. baseline, effects that were reverted in groups AC, IA, and IAC. The phosphorylation of protein kinase B (AKT) was higher in groups I and IA vs. groups KH and A. The phosphorylation of AMP-activated protein kinase (AMPK) was ∼31% higher in groups I, IA, and IAC vs. groups KH, A, and AC. The tert-butyl hydroperoxide (tBOOH)-induced chemiluminescence was lower (∼2.2 times) in all groups vs. group KH and was ∼35% lower in group IA vs. group A. Superoxide dismutase content was lower in groups A, AC, and IAC vs. group KH. Catalase activity was ∼28% lower in all groups (except group IA) vs. group KH. During reperfusion of the ischemic heart, insulin activates the AKT and AMPK pathways and inhibits the deleterious effects of angiotensin-I perfusion on SOD expression and cardiac function. The addition of captopril does not potentiate these effects. |
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Authors:
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Ubirajara Oliveira de Oliveira; Adriane Belló-Kein; Alvaro Reischak de Oliveira; Luiz Carlos Kuchaski; Ubiratan Fabres Machado; Maria Claudia Irigoyen; Beatriz D'Agord Schaan |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-10-26 |
Journal Detail:
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Title: Fundamental & clinical pharmacology Volume: - ISSN: 1472-8206 ISO Abbreviation: - Publication Date: 2011 Oct |
Date Detail:
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Created Date: 2011-10-27 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8710411 Medline TA: Fundam Clin Pharmacol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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© 2011 The Authors Fundamental and Clinical Pharmacology © 2011 Société Française de Pharmacologie et de Thérapeutique. |
Affiliation:
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Laboratory of Cardiovascular Physiology, Department of Physiology, ICBS, UFRGS, Porto Alegre, RS, Brazil Laboratory of Metabolism and Comparative Endocrinology, Department of Physiology, ICBS, UFRGS, Porto Alegre, RS, Brazil Laboratory of Research of Exercise, Escola de Educação Física, UFRGS, Porto Alegre, RS, Brazil Department of Physiology and Biophysics, ICB, USP, São Paulo, SP, Brazil Laboratory of Experimental Hypertension, Hypertension Unit, Instituto do Coração, HC-FMUSP, São Paulo, SP, Brazil Department of Internal Medicine, Endocrine Division, HCPA, UFRGS, Porto Alegre, RS, Brazil. |
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