Document Detail


Insulin action on muscle protein kinetics and amino acid transport during recovery after resistance exercise.
MedLine Citation:
PMID:  10331397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have determined the individual and combined effects of insulin and prior exercise on leg muscle protein synthesis and degradation, amino acid transport, glucose uptake, and alanine metabolism. Normal volunteers were studied in the postabsorptive state at rest and about 3 h after a heavy leg resistance exercise routine. The leg arteriovenous balance technique was used in combination with stable isotopic tracers of amino acids and biopsies of the vastus lateralis muscle. Insulin was infused into a femoral artery to increase the leg insulin concentrations to high physiologic levels without substantively affecting the whole-body level. Protein synthesis and degradation were determined as rates of intramuscular phenylalanine utilization and appearance, and muscle fractional synthetic rate (FSR) was also determined. Leg blood flow was greater after exercise than at rest (P<0.05). Insulin accelerated blood flow at rest but not after exercise (P<0.05). The rates of protein synthesis and degradation were greater during the postexercise recovery (65+/-10 and 74+/-10 nmol x min(-1) x 100 ml(-1) leg volume, respectively) than at rest (30+/-7 and 46+/-8 nmol x min(-1) x 100 ml(-1) leg volume, respectively; P<0.05). Insulin infusion increased protein synthesis at rest (51+/-4 nmol x min(-1) x 100 ml(-1) leg volume) but not during the postexercise recovery (64+/-9 nmol x min(-1) x 100 ml(-1) leg volume; P<0.05). Insulin infusion at rest did not change the rate of protein degradation (48+/-3 nmol x min(-1) 100 ml(-1) leg volume). In contrast, insulin infusion after exercise significantly decreased the rate of protein degradation (52+/-9 nmol x min(-1) x 100 ml(-1) leg volume). The insulin stimulatory effects on inward alanine transport and glucose uptake were three times greater during the postexercise recovery than at rest (P<0.05). In contrast, the insulin effects on phenylalanine, leucine, and lysine transport were similar at rest and after exercise. In conclusion, the ability of insulin to stimulate glucose uptake and alanine transport and to suppress protein degradation in skeletal muscle is increased after resistance exercise. Decreased amino acid availability may limit the stimulatory effect of insulin on muscle protein synthesis after exercise.
Authors:
G Biolo; B D Williams; R Y Fleming; R R Wolfe
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Publication Detail:
Type:  Clinical Trial; Journal Article; Randomized Controlled Trial; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Diabetes     Volume:  48     ISSN:  0012-1797     ISO Abbreviation:  Diabetes     Publication Date:  1999 May 
Date Detail:
Created Date:  1999-05-27     Completed Date:  1999-05-27     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372763     Medline TA:  Diabetes     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  949-57     Citation Subset:  AIM; IM; S    
Affiliation:
Department of Internal Medicine, University of Texas Medical Branch, and the Shriners Burns Hospital, Galveston, USA.
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MeSH Terms
Descriptor/Qualifier:
Adult
Alanine / metabolism
Amino Acids / blood,  metabolism*
Biological Transport
Blood Flow Velocity
Exercise / physiology*
Femoral Artery
Glucose / metabolism
Humans
Insulin / administration & dosage,  blood,  pharmacology*
Kinetics
Leg / blood supply
Lysine / metabolism
Male
Muscle Proteins / biosynthesis,  metabolism*
Muscle, Skeletal / metabolism*
Grant Support
ID/Acronym/Agency:
DK-38010/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Muscle Proteins; 11061-68-0/Insulin; 50-99-7/Glucose; 56-41-7/Alanine; 56-87-1/Lysine

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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