| Insulin-induced electrophysiology changes in human pleura are mediated via its receptor. | |
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MedLine Citation:
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PMID: 20814548 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Insulin directly changes the sheep pleural electrophysiology. The aim of this study was to investigate whether insulin induces similar effects in human pleura, to clarify insulin receptor's involvement, and to demonstrate if glibenclamide (hypoglycemic agent) reverses this effect. METHODS: Human parietal pleural specimens were mounted in Ussing chambers. Solutions containing insulin or glibenclamide and insulin with anti-insulin antibody, anti-insulin receptor antibody, and glibenclamide were used. The transmesothelial resistance (R(TM)) was determined. Immunohistochemistry for the presence of Insulin Receptors (IRa, IRb) was also performed. RESULTS: Insulin increased R(TM) within 1st min (P = .016), when added mesothelially which was inhibited by the anti-insulin and anti-insulin receptor antibodies. Glibenclamide also eliminated the insulin-induced changes. Immunohistochemistry verified the presence of IRa and IRb. CONCLUSION: Insulin induces electrochemical changes in humans as in sheep via interaction with its receptor. This effect is abolished by glibenclamide. |
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Authors:
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V K Kouritas; M Ioannou; C N Foroulis; N Desimonas; K Evaggelopoulos; K I Gourgoulianis; P A Molyvdas; C Hatzoglou |
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Publication Detail:
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Type: Journal Article Date: 2010-08-12 |
Journal Detail:
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Title: Experimental diabetes research Volume: 2010 ISSN: 1687-5303 ISO Abbreviation: Exp Diabetes Res Publication Date: 2010 |
Date Detail:
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Created Date: 2010-09-03 Completed Date: 2010-12-14 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101274844 Medline TA: Exp Diabetes Res Country: United States |
Other Details:
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Languages: eng Pagination: 853176 Citation Subset: IM |
Affiliation:
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Department of Physiology, Medical School, University of Thessaly, Mezourlo, New Buildings, Larissa, Greece. kouritas@otenet.gr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Glyburide
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pharmacology Humans Insulin / pharmacology* Pleura / drug effects*, physiology Receptor, IGF Type 1 / physiology Receptor, Insulin / analysis, physiology* |
| Chemical | |
Reg. No./Substance:
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10238-21-8/Glyburide; 11061-68-0/Insulin; EC 2.7.10.1/Receptor, IGF Type 1; EC 2.7.10.1/Receptor, Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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