| Insulin controls the spatial distribution of GLUT4 on the cell surface through regulation of its postfusion dispersal. | |
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MedLine Citation:
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PMID: 20816091 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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While the glucose transporter-4 (GLUT4) is fundamental to insulin-regulated glucose metabolism, its dynamic spatial organization in the plasma membrane (PM) is unclear. Here, using multicolor TIRF microscopy in transfected adipose cells, we demonstrate that insulin regulates not only the exocytosis of GLUT4 storage vesicles but also PM distribution of GLUT4 itself. In the basal state, domains (clusters) of GLUT4 molecules in PM are created by an exocytosis that retains GLUT4 at the fusion site. Surprisingly, when insulin induces a burst of GLUT4 exocytosis, it does not merely accelerate this basal exocytosis but rather stimulates approximately 60-fold another mode of exocytosis that disperses GLUT4 into PM. In contradistinction, internalization of most GLUT4, regardless of insulin, occurs from pre-existing clusters via the subsequent recruitment of clathrin. The data fit a new kinetic model that features multifunctional clusters as intermediates of exocytosis and endocytosis. |
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Authors:
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Karin G Stenkula; Vladimir A Lizunov; Samuel W Cushman; Joshua Zimmerberg |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, N.I.H., Intramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell metabolism Volume: 12 ISSN: 1932-7420 ISO Abbreviation: Cell Metab. Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-09-06 Completed Date: 2010-11-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233170 Medline TA: Cell Metab Country: United States |
Other Details:
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Languages: eng Pagination: 250-9 Citation Subset: IM |
Copyright Information:
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2010 Elsevier Inc. All rights reserved. |
Affiliation:
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Experimental Diabetes, Metabolism, and Nutrition Section, Diabetes Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adipocytes
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cytology,
metabolism Animals Caveolins / metabolism Cell Membrane / drug effects, metabolism* Cells, Cultured Clathrin / metabolism Endocytosis / drug effects*, physiology Exocytosis / drug effects*, physiology Glucose Transporter Type 4 / genetics, metabolism* Insulin / pharmacology, physiology* Membrane Fusion / physiology* Models, Biological Rats Recombinant Fusion Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Caveolins; 0/Clathrin; 0/Glucose Transporter Type 4; 0/Recombinant Fusion Proteins; 11061-68-0/Insulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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