| Insulin alters the permeability of sheep pleura. | |
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MedLine Citation:
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PMID: 19856254 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: Insulin promotes ion transportation across epithelia, mainly kidneys, leading to water and electrolyte abnormalities, possibly causing 'insulin oedema syndrome', which rarely presents as pleural effusion. Direct stimulation of sheep pleura by insulin and the possible electrophysiology mechanisms involved were investigated. MATERIAL AND METHODS: Sheep visceral and parietal pleural specimens were mounted between Ussing chambers. Insulin solutions (10 (-9) to 10 (-5) M), L-NAME, Nitroprussid sodium, amiloride and ouabain were used. Trans-mesothelial Resistance was determined. Immunohistochemistry for presence of Insulin Receptors was performed. RESULTS: Insulin increased Trans-mesothelial Resistance within 1st minute when added mesothelially of visceral (p=0.008) and parietal pleura (p=0.046) for concentrations higher than 10 (-7) M. L-NAME or Nitroprussid sodium didn't but amiloride and ouabain inhibited insulin's effect. Immunohistochemistry revealed the presence of Insulin Receptors. CONCLUSION: Insulin changes the permeability of sheep pleura by altering its electrophysiology and may interfere in pleural effusion formation. Involvement of Insulin Receptors may be suggested. |
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Authors:
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V K Kouritas; C Hatzoglou; M Ioannou; K I Gourgoulianis; P A Molyvdas |
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Publication Detail:
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Type: Journal Article Date: 2009-10-23 |
Journal Detail:
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Title: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association Volume: 118 ISSN: 1439-3646 ISO Abbreviation: Exp. Clin. Endocrinol. Diabetes Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-10 Completed Date: 2010-08-10 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9505926 Medline TA: Exp Clin Endocrinol Diabetes Country: Germany |
Other Details:
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Languages: eng Pagination: 304-9 Citation Subset: IM |
Affiliation:
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Department of Physiology, Medical School, University of Thessaly, Larissa, Greece. kouritas@otenet.gr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amiloride
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pharmacology Animals Cell Membrane Permeability / drug effects Dose-Response Relationship, Drug Insulin / pharmacology* Kinetics NG-Nitroarginine Methyl Ester / pharmacology Ouabain / pharmacology Pleura / drug effects, physiology* Receptor, Insulin / physiology Sheep |
| Chemical | |
Reg. No./Substance:
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11061-68-0/Insulin; 2609-46-3/Amiloride; 50903-99-6/NG-Nitroarginine Methyl Ester; 630-60-4/Ouabain; EC 2.7.10.1/Receptor, Insulin |
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