Document Detail


Instrumentation for chemical cytometry.
MedLine Citation:
PMID:  10701276     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Capillary electrophoresis is ideally suited to chemical analysis of individual cells. Small mammalian somatic cells (approximately 15 microns in diameter) can be analyzed by injecting the intact cell into a capillary, lysing the cell, separating and detecting the cellular components, and reconditioning the capillary prior to the next injection. In this paper, we report on technical improvements to single-cell analysis. We designed an inexpensive multipurpose single-cell injector that facilitates the following: (i) monitoring of injection, (ii) reproducible pressure- or electrokinetic-driven injection of the cell, (iii) complete cell lysis by SDS within 30 s of injection, and (iv) pressure-driven capillary reconditioning. Furthermore, we report on the analysis of glycosylation and glycolysis in single human carcinoma cells (HT29 cell line). The reliability and quality of the analysis is confirmed by comparing electropherograms from single cells and those from purified cell extracts.
Authors:
S N Krylov; D A Starke; E A Arriaga; Z Zhang; N W Chan; M M Palcic; N J Dovichi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical chemistry     Volume:  72     ISSN:  0003-2700     ISO Abbreviation:  Anal. Chem.     Publication Date:  2000 Feb 
Date Detail:
Created Date:  2000-04-25     Completed Date:  2000-04-25     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370536     Medline TA:  Anal Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  872-7     Citation Subset:  IM    
Affiliation:
Department of Chemistry, University of Alberta, Edmonton, Canada.
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MeSH Terms
Descriptor/Qualifier:
Electrophoresis, Capillary / instrumentation*,  methods
Glycolysis
Glycosylation
HT29 Cells
Humans
Microscopy, Fluorescence

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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