Document Detail

Instrument for fluorescence sensing of circulating cells with diffuse light in mice in vivo.
MedLine Citation:
PMID:  22502573     Owner:  NLM     Status:  MEDLINE    
Accurate quantification of circulating cell populations in mice is important in many areas of preclinical biomedical research. Normally, this is done either by extraction and analysis of small blood samples or, more recently, by using microscopy-based in vivo fluorescence flow cytometry. We describe a new technological approach to this problem using detection of diffuse fluorescent light from relatively large blood vessels in vivo. The diffuse fluorescence flow cytometer (DFFC) uses a laser to illuminate a mouse limb and an array of optical fibers coupled to a high-sensitivity photomultiplier tube array operating in photon counting mode to detect weak fluorescence signals from cells. We first demonstrate that the DFFC instrument is capable of detecting fluorescent microspheres and Vybrant-DiD-labeled cells in a custom-made optical flow phantom with similar size, optical properties, linear flow rates, and autofluorescence as a mouse limb. We also present preliminary data demonstrating that the DFFC is capable of detecting circulating cells in nude mice in vivo. In principle, this device would allow interrogation of the whole blood volume of a mouse in minutes, with sensitivity improvement by several orders of magnitude compared to current approaches.
Eric Zettergren; Dwayne Vickers; Judith Runnels; Shashi K Murthy; Charles P Lin; Mark Niedre
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  17     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2012 Mar 
Date Detail:
Created Date:  2012-04-16     Completed Date:  2012-08-15     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  037001     Citation Subset:  IM    
Copyright Information:
© 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).
Northeastern University, Department of Electrical and Computer Engineering, Boston, Massachusetts 02115, USA.
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MeSH Terms
Flow Cytometry / instrumentation*,  methods*
Fluorescent Dyes / chemistry
Mice, Nude
Phantoms, Imaging
Sensitivity and Specificity
Signal Processing, Computer-Assisted*
Grant Support
R21 HL098750-01/HL/NHLBI NIH HHS
Reg. No./Substance:
0/Fluorescent Dyes

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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