Document Detail

Instant hepatic differentiation of human embryonic stem cells using activin A and a deleted variant of HGF.
MedLine Citation:
PMID:  17299990     Owner:  NLM     Status:  MEDLINE    
Human embryonic stem (hES) cells have the ability to differentiate into a variety of different cell lineages and potentially provide a source of differentiated cells for many therapeutic uses. Here we investigated an efficient method of hepatic differentiation from hES cells. A human ES cell line, KhES-1, was used and maintained by a nonfeeder method. KhES-1 cells were cultured for 5 days in the presence of human activin A (50 ng/ml) and then treated with a deleted variant of hepatocyte growth factor (dHGF) at 0, 100, or 500 ng/ml for 7 days. The resultant cells were biologically analyzed. The expression of the endodermal genes SOX17 and FOXA2 increased in KhES-1 cells after activin A treatment. In contrast, Oct4, a self-renewal undifferentiated marker, decreased in a time-dependent manner in KhES-1 cells. Following a 7-day treatment of the resultant cells with dHGF, especially at 500 ng/ml, KhES-1 cells showed an expression of the hepatic makers albumin, AFP, and CK18. Transitional electron microscopy showed well-developed glycogen rosettes and a gap junction in KhES-1 cells treated with 500 ng/ml of dHGF. We developed an efficient method to differentiate KhES-1 cells into hepatocyte-like cells in vitro using 50 ng/ml of activin A and 500 ng/ml of dHGF.
Yong Chen; Alejandro Soto-Gutierrez; Nalu Navarro-Alvarez; Jorge David Rivas-Carrillo; Tomoki Yamatsuji; Yasuhiro Shirakawa; Noriaki Tanaka; Hesham Basma; Ira J Fox; Naoya Kobayashi
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Cell transplantation     Volume:  15     ISSN:  0963-6897     ISO Abbreviation:  Cell Transplant     Publication Date:  2006  
Date Detail:
Created Date:  2007-02-15     Completed Date:  2007-04-19     Revised Date:  2007-04-20    
Medline Journal Info:
Nlm Unique ID:  9208854     Medline TA:  Cell Transplant     Country:  United States    
Other Details:
Languages:  eng     Pagination:  865-71     Citation Subset:  IM    
Department of Surgery, Okayama University Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan.
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MeSH Terms
Activins / pharmacology*
Albumins / metabolism
Ammonia / metabolism
Cell Differentiation / drug effects*
Cell Line
Dose-Response Relationship, Drug
Embryonic Stem Cells / cytology,  drug effects*,  ultrastructure
Glycogen / metabolism
Hepatocyte Growth Factor / genetics,  pharmacology*
Hepatocytes / drug effects,  metabolism,  ultrastructure
Lidocaine / metabolism
Microscopy, Electron, Transmission
Reg. No./Substance:
0/Albumins; 104625-48-1/Activins; 137-58-6/Lidocaine; 67256-21-7/Hepatocyte Growth Factor; 7664-41-7/Ammonia; 9005-79-2/Glycogen
Erratum In:
Cell Transplant. 2007;16(1):97
Note: Basma, Hesham [added]; Fox, Ira J [added]

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