Document Detail

Instability of long inverted repeats within mouse transgenes.
MedLine Citation:
PMID:  8605887     Owner:  NLM     Status:  MEDLINE    
Various sequences in the mammalian genomes are unstable. One class of sequence arrangement is long inverted repeats, which are known to be unstable in bacteria and yeast. While in mammals some evidence suggests that short inverted repeats (<10 bp long) may show instability, nothing is known about the stability of long inverted repeats. Here we describe two unrelated multicopy transgenes in the mouse (loci 109 and OX1-5), each of which contains a long inverted repeat that shows substantial mitotic instability. This instability also occurs in the germline so that mutant transgenes appear within pedigrees at a high frequency. The mutation processes acting at these two inverted repeats are complex and can involve insertion or deletion, and can result in stabilization of the transgene. At transgene 109 mutational events range from very small rearrangements at the centre of the inverted repeat to complete transgene deletion. In addition we show that the rates of mutation at the inverted repeat of transgene OX1-5 can vary between the male and female germlines and between inbred strains of mice, suggesting the possibility of a genetic analysis to identify loci that modulate inverted repeat instability.
A Collick; J Drew; J Penberth; P Bois; J Luckett; F Scaerou; A Jeffreys; W Reik
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The EMBO journal     Volume:  15     ISSN:  0261-4189     ISO Abbreviation:  EMBO J.     Publication Date:  1996 Mar 
Date Detail:
Created Date:  1996-05-17     Completed Date:  1996-05-17     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  8208664     Medline TA:  EMBO J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1163-71     Citation Subset:  IM    
Department of Genetics, University of Leicester, United Kingdom.
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MeSH Terms
Base Sequence
DNA / genetics
DNA Primers / genetics
Mice, Inbred BALB C
Mice, Inbred DBA
Mice, Transgenic
Molecular Sequence Data
Mutagenesis, Insertional
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid*
Sequence Deletion
Reg. No./Substance:
0/DNA Primers; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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