| Insights into the micromechanical properties of the metaphase spindle. | |
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MedLine Citation:
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PMID: 21703450 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The microtubule-based metaphase spindle is subjected to forces that act in diverse orientations and over a wide range of timescales. Currently, we cannot explain how this dynamic structure generates and responds to forces while maintaining overall stability, as we have a poor understanding of its micromechanical properties. Here, we combine the use of force-calibrated needles, high-resolution microscopy, and biochemical perturbations to analyze the vertebrate metaphase spindle's timescale- and orientation-dependent viscoelastic properties. We find that spindle viscosity depends on microtubule crosslinking and density. Spindle elasticity can be linked to kinetochore and nonkinetochore microtubule rigidity, and also to spindle pole organization by kinesin-5 and dynein. These data suggest a quantitative model for the micromechanics of this cytoskeletal architecture and provide insight into how structural and functional stability is maintained in the face of forces, such as those that control spindle size and position, and can result from deformations associated with chromosome movement. |
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Authors:
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Yuta Shimamoto; Yusuke T Maeda; Shin'ichi Ishiwata; Albert J Libchaber; Tarun M Kapoor |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Cell Volume: 145 ISSN: 1097-4172 ISO Abbreviation: Cell Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-06-27 Completed Date: 2011-08-30 Revised Date: 2011-09-26 |
Medline Journal Info:
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Nlm Unique ID: 0413066 Medline TA: Cell Country: United States |
Other Details:
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Languages: eng Pagination: 1062-74 Citation Subset: IM |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Laboratory of Chemistry and Cell Biology, The Rockefeller University, 1230 York Avenue, New York, NY 10065, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biomechanics Cell Extracts / chemistry Dyneins / physiology Elasticity Kinesin / physiology Metaphase* Microtubules / physiology Mitotic Spindle Apparatus / chemistry*, physiology* Ovum / chemistry Xenopus Proteins / physiology Xenopus laevis / physiology* |
| Grant Support | |
ID/Acronym/Agency:
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GM065933/GM/NIGMS NIH HHS; R01 GM065933-08/GM/NIGMS NIH HHS; R01 GM065933-09/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cell Extracts; 0/Eg5 protein, Xenopus; 0/Xenopus Proteins; EC 3.6.1.-/Kinesin; EC 3.6.4.2/Dyneins |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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