| Insights into the mechanism of magnetic particle assisted gene delivery. | |
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MedLine Citation:
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PMID: 20920614 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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In magnetic particle assisted gene delivery DNA is complexed with polymer-coated aggregated magnetic nanoparticles (AMNPs) to effect transfection. In vitro studies based on COS-7 cells were carried out using pEGFP-N1 and pMIR-REPORT-complexed, polyethylenimine (PEI)-coated iron oxide magnetic nanoparticles (MNPs). PEI-coated AMNPs (PEI-AMNPs) with average individual particle diameters of 8, 16 and 30nm were synthesized. Normal, reverse and retention magnetic transfection experiments and cell wounding assays were performed. Our results show that the optimum magnetic field yields maximum transfection efficiency with good viability. The results of the normal, reverse and retention magnetic transfection experiments show that the highest transfection efficiency was achieved in normal magnetic transfection mode due to clustering of the PEI-AMNPs on the cells. Cell wounding assay results suggest that the mechanism of magnetic transfection is endocytosis rather than cell wounding. |
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Authors:
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D Ang; Q V Nguyen; S Kayal; P R Preiser; R S Rawat; R V Ramanujan |
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Publication Detail:
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Type: Journal Article Date: 2011-01-22 |
Journal Detail:
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Title: Acta biomaterialia Volume: 7 ISSN: 1878-7568 ISO Abbreviation: Acta Biomater Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-01-31 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101233144 Medline TA: Acta Biomater Country: England |
Other Details:
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Languages: eng Pagination: 1319-26 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
Affiliation:
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School of Materials Science and Engineering, Nanyang Technological University, Singapore 639798, Singapore. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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