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Insight into the Role of Sugars in Bud Burst Under Light in the Rose.
MedLine Citation:
PMID:  22505690     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Bud burst is a decisive process in plant architecture that requires light in Rosa sp. This light effect was correlated with stimulation of sugar transport and metabolism in favor of buds outgrowth. We investigated whether sugars could act as signaling entities in the light- mediated regulation of vacuolar invertases and bud burst. Full-length cDNAs encoding two vacuolar invertases (RhVI1 and RhVI2) were isolated from buds. Unlike RhVI2, RhVI1 was preferentially expressed in bursting buds, and was upregulated in buds of beheaded plants exposed to light. To assess the importance of sugars in this process, the expression of RhVI1 and RhVI2 and the total vacuolar invertase activity were further characterized in buds cultured in vitro on 100 mM sucrose or mannitol under light or in darkness for 48 h. Unlike mannitol, sucrose promoted the stimulatory effect of light on both RhVI1 expression and vacuolar invertase activity. This upregulation of RhVI1 was rapid (after 6 h incubation) and was induced by as little as 10 mM sucrose or fructose. No effect of glucose was found. Interestingly, both 30 mM palatinose (non-metabolizable sucrose analog) and 5 mM psicose (non-metabolizable fructose analog) promoted the light-induced expression of RhVI1 and total vacuolar invertase activity. Sucrose, fructose, palatinose and psicose all promoted bursting of in vitro cultured buds under light. These findings indicate that soluble sugars contribute to the light effect on bud burst and vacuolar invertases, and can function as signaling entities.
Authors:
Amelie Rabot; Clemence Henry; Khaoula Ben Baaziz; Eric Mortreau; Wassim Azri; Jeremy Lothier; Latifa Hamama; Rachid Boummaza; Nathalie Leduc; Sandrine Pelleschi-Travier; José Le Gourrierec; Soulaiman Sakr
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-4-13
Journal Detail:
Title:  Plant & cell physiology     Volume:  -     ISSN:  1471-9053     ISO Abbreviation:  -     Publication Date:  2012 Apr 
Date Detail:
Created Date:  2012-4-16     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Agrocampus-Ouest, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France, IUniversité d'Angers, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49045 Angers, France, INRA, Institut de Recherche en Horticulture et Semences (INRA, Agrocampus-Ouest, Université d'Angers), SFR 149 QUASAV, F-49071 Beaucouzé, France.
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