Document Detail


Inside single cells: quantitative analysis with advanced optics and nanomaterials.
MedLine Citation:
PMID:  25430077     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Single-cell explorations offer a unique window to inspect molecules and events relevant to mechanisms and heterogeneity constituting the central dogma of biology. A large number of nucleic acids, proteins, metabolites, and small molecules are involved in determining and fine-tuning the state and function of a single cell at a given time point. Advanced optical platforms and nanotools provide tremendous opportunities to probe intracellular components with single-molecule accuracy, as well as promising tools to adjust single-cell activity. To obtain quantitative information (e.g., molecular quantity, kinetics, and stoichiometry) within an intact cell, achieving the observation with comparable spatiotemporal resolution is a challenge. For single-cell studies, both the method of detection and the biocompatibility are critical factors as they determine the feasibility, especially when considering live-cell analysis. Although a considerable proportion of single-cell methodologies depend on specialized expertise and expensive instruments, it is our expectation that the information content and implication will outweigh the costs given the impact on life science enabled by single-cell analysis. For further resources related to this article, please visit the WIREs website. Conflict of interest: The authors have declared no conflicts of interest for this article.
Authors:
Yi Cui; Joseph Irudayaraj
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-11-27
Journal Detail:
Title:  Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology     Volume:  -     ISSN:  1939-0041     ISO Abbreviation:  Wiley Interdiscip Rev Nanomed Nanobiotechnol     Publication Date:  2014 Nov 
Date Detail:
Created Date:  2014-11-28     Completed Date:  -     Revised Date:  2014-11-29    
Medline Journal Info:
Nlm Unique ID:  101508311     Medline TA:  Wiley Interdiscip Rev Nanomed Nanobiotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2014 Wiley Periodicals, Inc.
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