Document Detail

Insertional mutagenesis: a Swiss Army knife for functional genomics of Medicago truncatula.
MedLine Citation:
PMID:  15882655     Owner:  NLM     Status:  MEDLINE    
Legumes are second only to grasses in worldwide economic importance, and understanding their molecular genetics is vital to the breeding of important grain and forage legumes. Over the past decade, Medicago truncatula has been selected as a model plant in which to study biological processes that are unique and pertinent to legumes, and that cannot easily be studied in Arabidopsis. Here, we discuss the most common tools for introducing and analyzing genetic mutations in M. truncatula. Because transformation and regeneration are still bottlenecks in studying a legume species, large-scale insertional mutagenesis poses a major challenge in M. truncatula. We discuss the tobacco retrotransposon Tnt1 as a viable and attractive option for introducing multiple independent insertions per plant for saturation mutagenesis.
Million Tadege; Pascal Ratet; Kirankumar S Mysore
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review    
Journal Detail:
Title:  Trends in plant science     Volume:  10     ISSN:  1360-1385     ISO Abbreviation:  Trends Plant Sci.     Publication Date:  2005 May 
Date Detail:
Created Date:  2005-05-10     Completed Date:  2005-06-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9890299     Medline TA:  Trends Plant Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  229-35     Citation Subset:  IM    
Plant Biology Division, The Samuel Roberts Noble Foundation, 2510 Sam Noble Parkway, Ardmore, OK 73401, USA.
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MeSH Terms
Arabidopsis / genetics
Gamma Rays
Gene Silencing
Genome, Plant*
Medicago / genetics*,  radiation effects
Models, Biological
Mutagenesis, Insertional*
Reg. No./Substance:

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