Document Detail

Insecticide resistance monitoring of field-collected Anopheles gambiae s.l. populations from Jinja, eastern Uganda, identifies high levels of pyrethroid resistance.
MedLine Citation:
PMID:  23046446     Owner:  NLM     Status:  MEDLINE    
Insecticide resistance in the malaria vector Anopheles gambiae s.l. (Diptera: Culicidae) threatens insecticide-based control efforts, necessitating regular monitoring. We assessed resistance in field-collected An. gambiae s.l. from Jinja, Uganda using World Health Organization (WHO) bioassays. Only An. gambiae s.s. and An. arabiensis (≈70%) were present. Female An. gambiae exhibited extremely high pyrethroid resistance (permethrin LT50 > 2 h; deltamethrin LT50 > 5 h). Female An. arabiensis were resistant to permethrin and exhibited reduced susceptibility to deltamethrin. However, while An. gambiae were DDT resistant, An. arabiensis were fully susceptible. Both species were fully susceptible to bendiocarb and fenitrothion. Kdr 1014S has increased rapidly in the Jinja population of An. gambiae s.s. and now approaches fixation (≈95%), consistent with insecticide-mediated selection, but is currently at a low frequency in An. arabiensis (0.07%). Kdr 1014F was also at a low frequency in An. gambiae. These frequencies preclude adequately-powered tests for an association with phenotypic resistance. PBO synergist bioassays resulted in near complete recovery of pyrethroid susceptibility suggesting involvement of CYP450s in resistance. A small number (0.22%) of An. gambiae s.s. ×An. arabiensis hybrids were found, suggesting the possibility of introgression of resistance alleles between species. The high levels of pyrethroid resistance encountered in Jinja threaten to reduce the efficacy of vector control programmes which rely on pyrethroid-impregnated bednets or indoor spraying of pyrethroids.
H D Mawejje; C S Wilding; E J Rippon; A Hughes; D Weetman; M J Donnelly
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-10-10
Journal Detail:
Title:  Medical and veterinary entomology     Volume:  27     ISSN:  1365-2915     ISO Abbreviation:  Med. Vet. Entomol.     Publication Date:  2013 Sep 
Date Detail:
Created Date:  2013-08-13     Completed Date:  2014-03-28     Revised Date:  2014-09-02    
Medline Journal Info:
Nlm Unique ID:  8708682     Medline TA:  Med Vet Entomol     Country:  England    
Other Details:
Languages:  eng     Pagination:  276-83     Citation Subset:  IM    
Copyright Information:
© 2012 The Royal Entomological Society.
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MeSH Terms
Anopheles gambiae / drug effects*,  genetics
Cytochrome P-450 Enzyme System / genetics,  metabolism
Insect Proteins / genetics,  metabolism
Insecticide Resistance*
Insecticides / pharmacology*
Lethal Dose 50
Polymerase Chain Reaction
Grant Support
Reg. No./Substance:
0/Insect Proteins; 0/Insecticides; 9035-51-2/Cytochrome P-450 Enzyme System

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